{"id":"26dafbf6-f66d-5a81-824d-ef34d924fe55","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-pineal-release","predicate":"increases_tested","statement":"The study reported D-aspartate-evoked glutamate secretion with blocker sensitivity similar to L-aspartate, supporting calcium-dependent vesicular release downstream of transport.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"17eb15e5-b0c2-51ed-a76e-f94b15714126","mechanism_event_label":"An aspartate signal can release another signaling amino acid.","subject":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"object":{"id":"230d2e32-1007-5317-b775-fb36bdd3d543","slug":"rat-pineal-glutamate-release","display_name":"Glutamate exocytosis from rat pinealocytes","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"17eb15e5-b0c2-51ed-a76e-f94b15714126","stable_key":"9a47f338-d127-5e4d-abf6-e99056833a69:d-aspartate-pineal-release-event","event_type":"observed_relationship","label":"An aspartate signal can release another signaling amino acid.","description":"The study reported D-aspartate-evoked glutamate secretion with blocker sensitivity similar to L-aspartate, supporting calcium-dependent vesicular release downstream of transport.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b3404670-6db1-517f-b9a0-27ecfaac558b","slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"230d2e32-1007-5317-b775-fb36bdd3d543","slug":"rat-pineal-glutamate-release","display_name":"Glutamate exocytosis from rat pinealocytes","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3078f0ee-56af-5480-b0a0-b0d6d108dd50","slug":"rat-pineal-calcium-entry","display_name":"Calcium entry in rat pinealocytes","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text; supplementary result described in main text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat pinealocytes; HPLC and calcium/exocytosis experiments; D-form comparison reported in supplemental results.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Main figures often use L-aspartate. Direct D-aspartate binding to inhibitory metabotropic receptors was not established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"D-Aspartate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"d-aspartate","display_name":"D-Aspartate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An aspartate signal can release another signaling amino acid.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Glutamate transporter-mediated glutamate secretion in the mammalian pineal gland. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18945893/ · DOI 10.1523/JNEUROSCI.0894-08.2008","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c49ad39b-e7b7-59d3-af6a-f3b663dc080a","evidence_kind":"source_excerpt","locator":"Lines 168-174","start_line":168,"end_line":174,"excerpt":"## d-aspartate-pineal-release\nAn aspartate signal can release another signaling amino acid.\nThe study reported D-aspartate-evoked glutamate secretion with blocker sensitivity similar to L-aspartate, supporting calcium-dependent vesicular release downstream of transport.\nModel: Rat pinealocytes; HPLC and calcium/exocytosis experiments; D-form comparison reported in supplemental results.\nLimitations: Main figures often use L-aspartate. Direct D-aspartate binding to inhibitory metabotropic receptors was not established.\nEvidence access: Primary full text; supplementary result described in main text\nGlutamate transporter-mediated glutamate secretion in the mammalian pineal gland. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18945893/ · DOI 10.1523/JNEUROSCI.0894-08.2008","model_system":"Rat pinealocytes; HPLC and calcium/exocytosis experiments; D-form comparison reported in supplemental results.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3751103e-e256-5615-b24f-39cc76ccfb47","stable_key":"import-9a47f338-d127-5e4d-abf6-e99056833a69","title":"D-Aspartate: synthesis, clearance, neural and endocrine mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"0a86a1008edb8a69dcc79409e76e3fd5ce59b0747be8cae8f21e43f871e76248","revision_id":"a1de4b66-263d-5452-8ed8-df853afba030","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}