{"id":"26ca46a2-688d-5892-a18a-c0838617d3d1","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:minus-lariciresinol-lowers-hepatitis-b-rna","predicate":"reduces","statement":"(−)-Lariciresinol blocked hepatitis B virus RNA production first, followed by viral proteins, then viral particles and DNA.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"343b7171-0aae-5aa8-b5fe-165f95bcedc3","mechanism_event_label":"(−)-Lariciresinol blocked hepatitis B virus RNA production first, followed by viral proteins, then viral particles and DNA.","subject":{"id":"bf9b95e9-f2d1-52bc-b68b-68f541085aeb","slug":"minus-lariciresinol","display_name":"(-)-Lariciresinol","entity_type_key":"small_molecule"},"object":{"id":"b6b0e047-df6c-5f77-a751-51019c928484","slug":"hbv-rna-production","display_name":"Hepatitis B virus RNA production","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"343b7171-0aae-5aa8-b5fe-165f95bcedc3","stable_key":"96d9034a-2550-5f3c-b767-1767c982533c:minus-lariciresinol-lowers-hepatitis-b-rna-event","event_type":"observed_relationship","label":"(−)-Lariciresinol blocked hepatitis B virus RNA production first, followed by viral proteins, then viral particles and DNA.","description":"(−)-Lariciresinol blocked hepatitis B virus RNA production first, followed by viral proteins, then viral particles and DNA.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bf9b95e9-f2d1-52bc-b68b-68f541085aeb","slug":"minus-lariciresinol","display_name":"(-)-Lariciresinol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b6b0e047-df6c-5f77-a751-51019c928484","slug":"hbv-rna-production","display_name":"Hepatitis B virus RNA production","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c9b0f8e4-4823-5067-a352-61b4c37cd11b","slug":"hnf1a","display_name":"Human hepatocyte nuclear factor 1 alpha / HNF1A","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Time course, order established by sequence of loss","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Hepatoma cell lines carrying hepatitis B virus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"(−)-Lariciresinol isolated from the roots of Isatis indigotica","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The order of events is what the time course establishes; the abstract does not give the concentrations at which each step was measured.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Hepatoma cell lines carrying hepatitis B virus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"(−)-Lariciresinol blocked hepatitis B virus RNA production first, followed by viral proteins, then viral particles and DNA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Anti-Hepatitis B Virus Activity of (-)-Lariciresinol Isolated from the Roots of Isatis indigotica. (2022). https://pubmed.ncbi.nlm.nih.gov/35630700/ DOI: 10.3390/molecules27103180","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Viral RNA, protein and particle production in order of appearance","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4642322e-242a-5e46-9a10-fdb541db459c","evidence_kind":"source_excerpt","locator":"Lines 77-86","start_line":77,"end_line":86,"excerpt":"## minus-lariciresinol-lowers-hepatitis-b-rna\n(−)-Lariciresinol blocked hepatitis B virus RNA production first, followed by viral proteins, then viral particles and DNA.\nModel/species: Hepatoma cell lines carrying hepatitis B virus\nTissue/system: Viral RNA, protein and particle production in order of appearance\nExposure: (−)-Lariciresinol isolated from the roots of Isatis indigotica\nRoute: In vitro\nDuration: Time course, order established by sequence of loss\nLimits: The order of events is what the time course establishes; the abstract does not give the concentrations at which each step was measured.\nPrimary reference: Anti-Hepatitis B Virus Activity of (-)-Lariciresinol Isolated from the Roots of Isatis indigotica. (2022). https://pubmed.ncbi.nlm.nih.gov/35630700/ DOI: 10.3390/molecules27103180\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"48c58761-0291-5bad-a926-75e904b2d802","stable_key":"import-96d9034a-2550-5f3c-b767-1767c982533c","title":"Lariciresinol: five molecules under one name, and the mechanisms each one carries (2026-09-22)","document_type":"imported_text","citation_label":"Original AI-assisted curation of twelve primary studies, every abstract read and all DOIs cross-checked against live PubMed metadata. Mechanism edges only, with no conclusion or claim of benefit recorded. Three author clusters account for eight of the twelve and carry shared laboratory keys. Study-specific concentrations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"6bf0015b7ff727b7a80d693dd18ffdd227d0e16ae45d9e85a5f81fa733edd3a4","revision_id":"219d9b0c-1a71-5e27-8d6c-7b3e77a594c6","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}