{"id":"26a17d54-059c-50f5-abd1-c03d28227767","stable_key":"911fb3c7-8cc3-5667-b677-5682fab67648:histidine-lat1-copper-complex","predicate":"transports_tested","statement":"Purified human LAT1 transported Cu(His)2 without the internal counter-substrate required for ordinary amino-acid antiport.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"9eba564d-33ce-51d3-b139-ef882dbc23ff","mechanism_event_label":"Binding copper changed how the histidine-containing species crossed this transporter.","subject":{"id":"16d078ff-692c-50eb-a34b-c61ef43821e1","slug":"slc7a5","display_name":"Human L-type amino acid transporter 1 / LAT1 / SLC7A5","entity_type_key":"protein"},"object":{"id":"cd41e4ab-b397-5acd-92fa-7474942659bc","slug":"copper-ii-bis-l-histidinate","display_name":"Copper(II) bis(L-histidinate) / Cu(His)2","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"9eba564d-33ce-51d3-b139-ef882dbc23ff","stable_key":"911fb3c7-8cc3-5667-b677-5682fab67648:histidine-lat1-copper-complex-event","event_type":"observed_relationship","label":"Binding copper changed how the histidine-containing species crossed this transporter.","description":"Purified human LAT1 transported Cu(His)2 without the internal counter-substrate required for ordinary amino-acid antiport.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"16d078ff-692c-50eb-a34b-c61ef43821e1","slug":"slc7a5","display_name":"Human L-type amino acid transporter 1 / LAT1 / SLC7A5","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cd41e4ab-b397-5acd-92fa-7474942659bc","slug":"copper-ii-bis-l-histidinate","display_name":"Copper(II) bis(L-histidinate) / Cu(His)2","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"44374451-3436-5136-a8ae-5dcc6d8c353b","slug":"histidine","display_name":"L-Histidine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9f0afdde-1ec1-5c8a-bb5e-f3b2b75f67f6","slug":"copper","display_name":"Copper","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Reconstituted human LAT1; radiotracer uptake, copper mass spectrometry and mutagenesis; representative assay 40 micromolar histidine plus 20 micromolar copper sulfate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This in-vitro uniport finding does not establish human copper delivery, safety or treatment efficacy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Histidine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"histidine","display_name":"L-Histidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Binding copper changed how the histidine-containing species crossed this transporter.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"LAT1 (SLC7A5) catalyzes copper(histidinate) transport switching from antiport to uniport mechanism. · 2023 · https://pubmed.ncbi.nlm.nih.gov/37692288/ · DOI 10.1016/j.isci.2023.107738","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Transported without the internal counter-substrate ordinary antiport needs, so this record is a uniport measurement.","entity":null},{"dimension":"transport_pool","value_text":"the proteoliposome interior","comparator":null,"unit":null,"notes":"Transported without the internal counter-substrate ordinary antiport needs, so this record is a uniport measurement.","entity":null}],"evidence":[{"id":"17eb31a8-3155-5e15-b3bf-6109a02993a3","evidence_kind":"source_excerpt","locator":"Lines 82-88","start_line":82,"end_line":88,"excerpt":"## histidine-lat1-copper-complex\nBinding copper changed how the histidine-containing species crossed this transporter.\nPurified human LAT1 transported Cu(His)2 without the internal counter-substrate required for ordinary amino-acid antiport.\nModel: Reconstituted human LAT1; radiotracer uptake, copper mass spectrometry and mutagenesis; representative assay 40 micromolar histidine plus 20 micromolar copper sulfate.\nLimitations: This in-vitro uniport finding does not establish human copper delivery, safety or treatment efficacy.\nEvidence access: Primary full text\nLAT1 (SLC7A5) catalyzes copper(histidinate) transport switching from antiport to uniport mechanism. · 2023 · https://pubmed.ncbi.nlm.nih.gov/37692288/ · DOI 10.1016/j.isci.2023.107738","model_system":"Reconstituted human LAT1; radiotracer uptake, copper mass spectrometry and mutagenesis; representative assay 40 micromolar histidine plus 20 micromolar copper sulfate.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"ce59e6c9-1213-523b-9d0b-400b7a81cd1c","stable_key":"import-911fb3c7-8cc3-5667-b677-5682fab67648","title":"L-Histidine: supply, catabolism, histamine, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"cb672530ec8b4a4542d0f3957e80ca76bb0449e9f988845b0f7681f623c4ec9b","revision_id":"19a7f26c-d9a1-5411-a81d-025f3d98a452","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}