{"id":"269afd69-f226-54e8-ad5d-3cf77955df22","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-adipocyte-glut4","predicate":"reduces_expression","statement":"During mouse 3T3-L1 differentiation, fisetin reduced GLUT4 expression and glucose uptake.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"580d0c44-f21d-53c5-9ff7-86be1ef8b3f9","mechanism_event_label":"Less glucose entry reduced lipid accumulation in this model.","subject":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"object":{"id":"66d34dc0-d0fc-5ebb-9d7c-dc88f6b47cea","slug":"mouse-slc2a4","display_name":"Mouse glucose transporter 4 / Slc2a4","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"580d0c44-f21d-53c5-9ff7-86be1ef8b3f9","stable_key":"74ef5bba-ee14-531c-b1bf-d1f297c56bb6:fisetin-adipocyte-glut4-event","event_type":"observed_relationship","label":"Less glucose entry reduced lipid accumulation in this model.","description":"During mouse 3T3-L1 differentiation, fisetin reduced GLUT4 expression and glucose uptake.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"dee05061-c3f3-5226-8054-dd58b76a585d","slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"66d34dc0-d0fc-5ebb-9d7c-dc88f6b47cea","slug":"mouse-slc2a4","display_name":"Mouse glucose transporter 4 / Slc2a4","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Differentiating mouse adipocytes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is not evidence that fisetin improves glucose uptake in human muscle.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fisetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fisetin","display_name":"Fisetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Less glucose entry reduced lipid accumulation in this model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Fisetin Suppresses Lipid Accumulation in Mouse Adipocytic 3T3-L1 Cells by Repressing GLUT4-Mediated Glucose Uptake through Inhibition of mTOR-C/EBPα Signaling. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25945786/ · DOI 10.1021/acs.jafc.5b00821","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a3a90fc8-c843-5b98-838c-b0dd4fa73fbc","evidence_kind":"source_excerpt","locator":"Lines 296-302","start_line":296,"end_line":302,"excerpt":"## fisetin-adipocyte-glut4\nLess glucose entry reduced lipid accumulation in this model.\nDuring mouse 3T3-L1 differentiation, fisetin reduced GLUT4 expression and glucose uptake.\nModel: Differentiating mouse adipocytes.\nLimitations: This is not evidence that fisetin improves glucose uptake in human muscle.\nEvidence access: Primary abstract\nFisetin Suppresses Lipid Accumulation in Mouse Adipocytic 3T3-L1 Cells by Repressing GLUT4-Mediated Glucose Uptake through Inhibition of mTOR-C/EBPα Signaling. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25945786/ · DOI 10.1021/acs.jafc.5b00821","model_system":"Differentiating mouse adipocytes.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"1108bfff-6504-5152-a51f-ff3d7c27c282","stable_key":"import-74ef5bba-ee14-531c-b1bf-d1f297c56bb6","title":"Fisetin: metabolism, cell-state responses and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c30e0764cd34bd062cfcbfaf32357ef29dfda30e7d4153c545178140f5054e09","revision_id":"40ff52c0-f4b9-5820-8abb-ff002c6364a4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}