{"id":"26712863-e674-51de-8bc0-595d1f33377a","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:hka-sensitive-proton-secretion","predicate":"supports","statement":"Luminal omeprazole abolished net total-CO2 flux used to measure acidification in collecting ducts from potassium-restricted rabbits.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"95d36abb-8ab6-5e50-849f-bbe75d760cc1","mechanism_event_label":"An H/K-pump-compatible activity supported acid secretion in the isolated duct.","subject":{"id":"77a0b92b-ccdd-5ab2-a032-0ac8fc8f93d5","slug":"renal-hydrogen-potassium-atpases","display_name":"Renal hydrogen-potassium ATPase activities","entity_type_key":"protein_family"},"object":{"id":"744b874b-7b8d-541d-a738-4ef7b627dc11","slug":"collecting-duct-proton-secretion","display_name":"Collecting duct proton secretion","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"95d36abb-8ab6-5e50-849f-bbe75d760cc1","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:hka-sensitive-proton-secretion-event","event_type":"biochemical_relationship","label":"An H/K-pump-compatible activity supported acid secretion in the isolated duct.","description":"Luminal omeprazole abolished net total-CO2 flux used to measure acidification in collecting ducts from potassium-restricted rabbits.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"77a0b92b-ccdd-5ab2-a032-0ac8fc8f93d5","slug":"renal-hydrogen-potassium-atpases","display_name":"Renal hydrogen-potassium ATPase activities","entity_type_key":"protein_family"},"role":"upstream","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"744b874b-7b8d-541d-a738-4ef7b627dc11","slug":"collecting-duct-proton-secretion","display_name":"Collecting duct proton secretion","entity_type_key":"cellular_process"},"role":"downstream","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"16a9848a-023b-5828-9fd1-e28b9eadb901","slug":"hydrogen-ion","display_name":"Hydrogen ion","entity_type_key":"ion"},"role":"secreted-ion","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"endpoint","value_text":"Luminal omeprazole abolished net total-CO2 flux used to measure acidification in collecting ducts from potassium-restricted rabbits.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental-exposure","value_text":"Microperfused inner-stripe outer medullary collecting ducts from rabbits fed 0.55% potassium diet for 7-14 days; luminal omeprazole 0.1 mM.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Microperfused inner-stripe outer medullary collecting ducts from rabbits fed 0.55% potassium diet for 7-14 days; luminal omeprazole 0.1 mM.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Pharmacological activity assignment; the experiment does not isolate ATP4A versus ATP12A. Total-CO2 flux is an acidification surrogate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Oryctolagus cuniculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"An H/K-pump-compatible activity supported acid secretion in the isolated duct.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[wingo-1989-hka] Active proton secretion and potassium absorption in the rabbit outer medullary collecting duct. Functional evidence for proton-potassium-activated adenosine triphosphatase (1989). https://pubmed.ncbi.nlm.nih.gov/2544629/ DOI: 10.1172/JCI114165","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"inner-stripe outer medullary collecting duct","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_direction","value_text":"potassium: tubular lumen toward blood; protons: cell toward tubular lumen","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"06efdb34-96ce-5d71-ac42-90d7266722cb","evidence_kind":"source_excerpt","locator":"Lines 1096-1108","start_line":1096,"end_line":1108,"excerpt":"### hka-sensitive-proton-secretion\nLuminal omeprazole abolished net total-CO2 flux used to measure acidification in collecting ducts from potassium-restricted rabbits.\nCondition category: nutrient_deficiency\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: An H/K-pump-compatible activity supported acid secretion in the isolated duct.\norganism: Oryctolagus cuniculus\ntissue_or_cell_type: inner-stripe outer medullary collecting duct\nexperimental_model: Microperfused inner-stripe outer medullary collecting ducts from rabbits fed 0.55% potassium diet for 7-14 days; luminal omeprazole 0.1 mM.\nlimitations: Pharmacological activity assignment; the experiment does not isolate ATP4A versus ATP12A. Total-CO2 flux is an acidification surrogate.\ntransport_direction: potassium: tubular lumen toward blood; protons: cell toward tubular lumen\nexperimental-exposure: Microperfused inner-stripe outer medullary collecting ducts from rabbits fed 0.55% potassium diet for 7-14 days; luminal omeprazole 0.1 mM.\nendpoint: Luminal omeprazole abolished net total-CO2 flux used to measure acidification in collecting ducts from potassium-restricted rabbits.\n[wingo-1989-hka] Active proton secretion and potassium absorption in the rabbit outer medullary collecting duct. Functional evidence for proton-potassium-activated adenosine triphosphatase (1989). https://pubmed.ncbi.nlm.nih.gov/2544629/ DOI: 10.1172/JCI114165","model_system":"Microperfused inner-stripe outer medullary collecting ducts from rabbits fed 0.55% potassium diet for 7-14 days; luminal omeprazole 0.1 mM.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [wingo-1989-hka] Active proton secretion and potassium absorption in the rabbit outer medullary collecting duct. Functional evidence for proton-potassium-activated adenosine triphosphatase (1989). https://pubmed.ncbi.nlm.nih.gov/2544629/ DOI: 10.1172/JCI114165","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}