{"id":"2670d8a3-4b4c-5eb8-9a03-5b98fc14a4dd","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-lat2-exchange","predicate":"exchanges","statement":"Human LAT2/SLC3A2 transported methionine; leucine and phenylalanine stimulated methionine efflux even against an inward methionine gradient.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a1f6f6c4-106b-5907-a813-25a74158f1fa","mechanism_event_label":"Transport depends on substrates on both sides of the membrane.","subject":{"id":"02373ade-38d4-50e0-b74d-f7e9ab0e3846","slug":"slc7a8","display_name":"Human L-type amino acid transporter 2 / LAT2 / SLC7A8","entity_type_key":"protein"},"object":{"id":"9d39f561-740b-5f67-bba7-8a72ef612a99","slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"a1f6f6c4-106b-5907-a813-25a74158f1fa","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-lat2-exchange-event","event_type":"observed_relationship","label":"Transport depends on substrates on both sides of the membrane.","description":"Human LAT2/SLC3A2 transported methionine; leucine and phenylalanine stimulated methionine efflux even against an inward methionine gradient.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"02373ade-38d4-50e0-b74d-f7e9ab0e3846","slug":"slc7a8","display_name":"Human L-type amino acid transporter 2 / LAT2 / SLC7A8","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9d39f561-740b-5f67-bba7-8a72ef612a99","slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"1ca4b4d5-616a-54d3-8244-0dc151b1bcfe","slug":"slc3a2","display_name":"4F2hc / SLC3A2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f707c753-cc8c-5f06-90be-bb3d73c60a18","slug":"leucine","display_name":"L-Leucine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"190407ad-0219-54b5-b05f-7c75f3895ca6","slug":"l-phenylalanine","display_name":"L-Phenylalanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human transporter in Xenopus oocytes; tracer influx and efflux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not show that ordinary mixed meals deplete methionine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Methionine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Transport depends on substrates on both sides of the membrane.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Transport of a neurotoxicant by molecular mimicry: the methylmercury-L-cysteine complex is a substrate for human L-type large neutral amino acid transporter (LAT) 1 and LAT2. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12117417/ · DOI 10.1042/BJ20020841","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"264e9412-bf27-5542-b85e-0ab092d91ef0","evidence_kind":"source_excerpt","locator":"Lines 28-34","start_line":28,"end_line":34,"excerpt":"## methionine-lat2-exchange\nTransport depends on substrates on both sides of the membrane.\nHuman LAT2/SLC3A2 transported methionine; leucine and phenylalanine stimulated methionine efflux even against an inward methionine gradient.\nModel: Human transporter in Xenopus oocytes; tracer influx and efflux.\nLimitations: Does not show that ordinary mixed meals deplete methionine.\nEvidence access: Primary abstract\nTransport of a neurotoxicant by molecular mimicry: the methylmercury-L-cysteine complex is a substrate for human L-type large neutral amino acid transporter (LAT) 1 and LAT2. · 2002 · https://pubmed.ncbi.nlm.nih.gov/12117417/ · DOI 10.1042/BJ20020841","model_system":"Human transporter in Xenopus oocytes; tracer influx and efflux.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d969b004-ccc2-5bb4-9109-8ac649137ad1","stable_key":"import-41a89233-da9a-5b7b-9c18-e19bbadcfe2d","title":"L-Methionine: transport, methylation, sulfur metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"f1c706ef72932eed85df234b6c3304158d503d626a63c69b02a453c162700d26","revision_id":"549035e0-3a72-550a-8a55-e8c5baa51b0d","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}