{"id":"262db9fc-04f6-52b4-8431-4162fdb83cfb","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-d-egf-binding","predicate":"interferes_in_tested_ligand_binding_to","statement":"Cucurbitacin D interfered with EGF–EGFR binding in a solid-phase binding assay and reduced EGFR phosphorylation in the NSCLC study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"79c722aa-526e-59b5-8798-f2f41c3461c0","mechanism_event_label":"A growth-factor interaction was tested upstream of signaling.","subject":{"id":"13edf322-5d31-5a4e-802f-c7528fc2ef48","slug":"cucurbitacin-d","display_name":"Cucurbitacin D","entity_type_key":"small_molecule"},"object":{"id":"844ed9d4-1202-5e1f-8db2-771dce1b0b3d","slug":"egfr","display_name":"Epidermal growth factor receptor","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"79c722aa-526e-59b5-8798-f2f41c3461c0","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-d-egf-binding-event","event_type":"observed_relationship","label":"A growth-factor interaction was tested upstream of signaling.","description":"Cucurbitacin D interfered with EGF–EGFR binding in a solid-phase binding assay and reduced EGFR phosphorylation in the NSCLC study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"13edf322-5d31-5a4e-802f-c7528fc2ef48","slug":"cucurbitacin-d","display_name":"Cucurbitacin D","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"844ed9d4-1202-5e1f-8db2-771dce1b0b3d","slug":"egfr","display_name":"Epidermal growth factor receptor","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Binding assay and gefitinib-resistant human NSCLC cell models.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This does not establish a clinically effective replacement for an EGFR inhibitor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"A growth-factor interaction was tested upstream of signaling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Cucurbitacin D Overcomes Gefitinib Resistance by Blocking EGF Binding to EGFR and Inducing Cell Death in NSCLCs. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32133284/ · DOI 10.3389/fonc.2020.00062","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"a017be3d-0582-5d38-ba14-0fd20dfffcaf","evidence_kind":"source_excerpt","locator":"Lines 332-338","start_line":332,"end_line":338,"excerpt":"## cucurbitacin-d-egf-binding\nA growth-factor interaction was tested upstream of signaling.\nCucurbitacin D interfered with EGF–EGFR binding in a solid-phase binding assay and reduced EGFR phosphorylation in the NSCLC study.\nModel: Binding assay and gefitinib-resistant human NSCLC cell models.\nLimitations: This does not establish a clinically effective replacement for an EGFR inhibitor.\nEvidence access: Primary abstract\nCucurbitacin D Overcomes Gefitinib Resistance by Blocking EGF Binding to EGFR and Inducing Cell Death in NSCLCs. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32133284/ · DOI 10.3389/fonc.2020.00062","model_system":"Binding assay and gefitinib-resistant human NSCLC cell models.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"5c23ec61b9cf3d2966fb081d8617e1e1f7c522e86419b8ba2dcae09590121123","revision_id":"74251a06-3ba5-506c-b428-700eca0fb432","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}