{"id":"26155be1-b4e6-5433-94ae-bab85a28f395","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-nac-cyp2d6","predicate":"inhibits_tested","statement":"N-acetyl-SAC inhibited the CYP2D6 probe reaction by 19% at 1 mM.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"95a78235-c3a8-53e5-8a2b-ac2906d52309","mechanism_event_label":"A metabolite differed from the parent.","subject":{"id":"0837bab7-ef2d-533f-9025-740a1c76cdf3","slug":"n-acetyl-s-allylcysteine","display_name":"N-acetyl-S-allyl-L-cysteine","entity_type_key":"small_molecule"},"object":{"id":"10f7a103-d63d-5bf4-8384-c5e1308ce126","slug":"cyp2d6","display_name":"Human cytochrome P450 2D6","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"95a78235-c3a8-53e5-8a2b-ac2906d52309","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-nac-cyp2d6-event","event_type":"observed_relationship","label":"A metabolite differed from the parent.","description":"N-acetyl-SAC inhibited the CYP2D6 probe reaction by 19% at 1 mM.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"0837bab7-ef2d-533f-9025-740a1c76cdf3","slug":"n-acetyl-s-allylcysteine","display_name":"N-acetyl-S-allyl-L-cysteine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"10f7a103-d63d-5bf4-8384-c5e1308ce126","slug":"cyp2d6","display_name":"Human cytochrome P450 2D6","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"acting_entity","value_text":"n-acetyl-s-allylcysteine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"SAC 0.01–1 mM; N-acetyl-SAC 1 mM","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Probe-specific incubation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary full-text HTML, abstract/results","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_comparison","value_text":"Test compound versus probe reaction without compound","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Pooled human liver microsomes; probe substrate metabolism","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived research curation; not independent primary verification","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Small in-vitro effect; no patient interaction magnitude.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"S-allylcysteine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human liver preparation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A metabolite differed from the parent.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[27725449] Evaluation of the Effects of S-Allyl-L-cysteine, S-Methyl-L-cysteine, trans-S-1-Propenyl-L-cysteine, and Their N-Acetylated and S-Oxidized Metabolites on Human CYP Activities. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27725449/ · DOI 10.1248/bpb.b16-00449","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Pooled human liver microsomes; probe substrate metabolism","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b100954d-38c5-545f-b935-4cefec54defd","evidence_kind":"source_excerpt","locator":"Lines 428-435","start_line":428,"end_line":435,"excerpt":"## s-allylcysteine-nac-cyp2d6\nA metabolite differed from the parent.\nN-acetyl-SAC inhibited the CYP2D6 probe reaction by 19% at 1 mM.\nModel: Pooled human liver microsomes; probe substrate metabolism\nLimitations: Small in-vitro effect; no patient interaction magnitude.\nEvidence access: Primary full-text HTML, abstract/results\n[27725449] Evaluation of the Effects of S-Allyl-L-cysteine, S-Methyl-L-cysteine, trans-S-1-Propenyl-L-cysteine, and Their N-Acetylated and S-Oxidized Metabolites on Human CYP Activities. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27725449/ · DOI 10.1248/bpb.b16-00449\nStructured context: {\"organism\": \"Human liver preparation\", \"tissue_or_cell_type\": \"Pooled human liver microsomes; probe substrate metabolism\", \"dose\": \"SAC 0.01–1 mM; N-acetyl-SAC 1 mM\", \"duration\": \"Probe-specific incubation\", \"route\": \"In vitro\", \"experimental_comparison\": \"Test compound versus probe reaction without compound\", \"acting_entity\": \"n-acetyl-s-allylcysteine\", \"interpretation_status\": \"Source-derived research curation; not independent primary verification\"}","model_system":"Pooled human liver microsomes; probe substrate metabolism","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3b0d58f9-8a69-579d-9920-518399a9a8bb","stable_key":"import-d96b230c-481b-535b-930c-04eb87ec7f02","title":"S-allylcysteine: sulfur signaling, redox responses and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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