{"id":"25dffabf-7fdb-5374-817d-fe6b34845910","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:va-repro-human-meiotic-panel-boundary","predicate":"does_not_significantly_increase","statement":"The same 24-hour RA exposure did not significantly increase SYCP3 or DMC1 RNA in human fetal testis cultures.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"44ebea80-23b0-5d2a-89ce-bfbf799391ed","mechanism_event_label":"STRA8 induction did not activate the full tested meiotic marker panel.","subject":{"id":"05487b29-8c59-5af8-83cf-cf9c79c5ecb8","slug":"all-trans-retinoic-acid","display_name":"All-trans-retinoic acid","entity_type_key":"small_molecule"},"object":{"id":"1240e977-9142-51dd-a72b-c0e07bbadf6a","slug":"human-meiotic-marker-expression","display_name":"Human meiotic marker expression","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"44ebea80-23b0-5d2a-89ce-bfbf799391ed","stable_key":"3c852f36-7951-5fd9-9013-d771a8d16d8a:va-repro-human-meiotic-panel-boundary-event","event_type":"biochemical_relationship","label":"STRA8 induction did not activate the full tested meiotic marker panel.","description":"The same 24-hour RA exposure did not significantly increase SYCP3 or DMC1 RNA in human fetal testis cultures.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8394f01d-62c5-5350-9e5e-60d7b0acc441","slug":"stra8","display_name":"Human STRA8","entity_type_key":"protein"},"role":"induced_but_insufficient_marker","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"05487b29-8c59-5af8-83cf-cf9c79c5ecb8","slug":"all-trans-retinoic-acid","display_name":"All-trans-retinoic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"1240e977-9142-51dd-a72b-c0e07bbadf6a","slug":"human-meiotic-marker-expression","display_name":"Human meiotic marker expression","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Paired cell-culture transcript assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"14-15 weeks gestation; 1 micromolar RA for 24 h; n=6.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A limited time and marker panel cannot prove permanent failure to enter meiosis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-a","display_name":"Vitamin A","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"STRA8 induction did not activate the full tested meiotic marker panel.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[human2011] Retinoic Acid signalling and the control of meiotic entry in the human fetal gonad. (2011). https://pubmed.ncbi.nlm.nih.gov/21674038/ DOI: 10.1371/journal.pone.0020249","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"human fetal testis","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3fc855f8-93b2-5452-830a-5cf19728fea8","evidence_kind":"source_excerpt","locator":"Lines 343-354","start_line":343,"end_line":354,"excerpt":"### va-repro-human-meiotic-panel-boundary\nThe same 24-hour RA exposure did not significantly increase SYCP3 or DMC1 RNA in human fetal testis cultures.\nCondition category: normal\nnutrient_topic: Vitamin A expansion research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: STRA8 induction did not activate the full tested meiotic marker panel.\norganism: Homo sapiens\ntissue_or_cell_type: human fetal testis\nexperimental_model: Paired cell-culture transcript assay\nlimitations: A limited time and marker panel cannot prove permanent failure to enter meiosis.\nexposure: 14-15 weeks gestation; 1 micromolar RA for 24 h; n=6.\ncross_nutrient: false\n[human2011] Retinoic Acid signalling and the control of meiotic entry in the human fetal gonad. 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