{"id":"25a2aa5e-e961-5de8-8d54-fb54db5f7cfd","stable_key":"0ad8610d-d575-5870-b7cd-763a9f750783:copper-cp-variant-apo","predicate":"g631r_variant_secreted_as","statement":"The aceruloplasminemia-associated G631R variant was synthesized and secreted as apoceruloplasmin in the cell-expression study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d25cb493-6715-5479-99f1-e3adbeeb4f7d","mechanism_event_label":"A copper-handling protein can be produced and released yet still lack its metal.","subject":{"id":"d372c77e-4e7d-5908-b597-57cfce485ed0","slug":"cp","display_name":"Human ceruloplasmin / CP","entity_type_key":"protein"},"object":{"id":"ab8d154a-6d53-5373-9c98-dd3084ea51e8","slug":"human-apoceruloplasmin","display_name":"Human ceruloplasmin without incorporated copper","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"d25cb493-6715-5479-99f1-e3adbeeb4f7d","stable_key":"0ad8610d-d575-5870-b7cd-763a9f750783:copper-cp-variant-apo-event","event_type":"biochemical_relationship","label":"A copper-handling protein can be produced and released yet still lack its metal.","description":"The aceruloplasminemia-associated G631R variant was synthesized and secreted as apoceruloplasmin in the cell-expression study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"d372c77e-4e7d-5908-b597-57cfce485ed0","slug":"cp","display_name":"Human ceruloplasmin / CP","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ab8d154a-6d53-5373-9c98-dd3084ea51e8","slug":"human-apoceruloplasmin","display_name":"Human ceruloplasmin without incorporated copper","entity_type_key":"protein_state"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/copper-research/12351628.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"4492680e4d410b1e32f77dea6aa0c26b2303b26d4fadfef25a350338ac365de3\", \"start_char\": 0, \"end_char\": 1541, \"text_sha256\": \"4492680e4d410b1e32f77dea6aa0c26b2303b26d4fadfef25a350338ac365de3\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human ceruloplasmin expression, radiocopper labeling and in-vitro loading","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Wild-type and copper-site mutants including G631R","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The final conformation required six occupied sites in these assays; no hierarchy was apparent. Do not confuse this structural requirement with circulating protein concentration.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Copper research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"copper","display_name":"Copper","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human protein expressed in Chinese hamster ovary cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A copper-handling protein can be produced and released yet still lack its metal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[copper-p12351628] Mechanisms of copper incorporation into human ceruloplasmin. (2002). https://pubmed.ncbi.nlm.nih.gov/12351628/ DOI: 10.1074/jbc.m206246200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Secretory protein biosynthesis","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"07b102cd-0baf-5a8d-a911-9e2cb772eaef","evidence_kind":"source_excerpt","locator":"Lines 1066-1077","start_line":1066,"end_line":1077,"excerpt":"### copper-cp-variant-apo\nThe aceruloplasminemia-associated G631R variant was synthesized and secreted as apoceruloplasmin in the cell-expression study.\nCondition category: normal\nnutrient_topic: Copper research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A copper-handling protein can be produced and released yet still lack its metal.\norganism: Human protein expressed in Chinese hamster ovary cells\ntissue_or_cell_type: Secretory protein biosynthesis\nexperimental_model: Human ceruloplasmin expression, radiocopper labeling and in-vitro loading\nlimitations: The final conformation required six occupied sites in these assays; no hierarchy was apparent. Do not confuse this structural requirement with circulating protein concentration.\nexposure: Wild-type and copper-site mutants including G631R\nevidence_span: {\"source_cache\": \"artifacts/copper-research/12351628.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"4492680e4d410b1e32f77dea6aa0c26b2303b26d4fadfef25a350338ac365de3\", \"start_char\": 0, \"end_char\": 1541, \"text_sha256\": \"4492680e4d410b1e32f77dea6aa0c26b2303b26d4fadfef25a350338ac365de3\"}\n[copper-p12351628] Mechanisms of copper incorporation into human ceruloplasmin. (2002). https://pubmed.ncbi.nlm.nih.gov/12351628/ DOI: 10.1074/jbc.m206246200","model_system":"Human ceruloplasmin expression, radiocopper labeling and in-vitro loading","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [copper-p12351628] Mechanisms of copper incorporation into human ceruloplasmin. (2002). https://pubmed.ncbi.nlm.nih.gov/12351628/ DOI: 10.1074/jbc.m206246200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9afba495-cbdc-51aa-998e-70a930dba3be","stable_key":"import-0ad8610d-d575-5870-b7cd-763a9f750783","title":"Copper: transport, cuproenzymes, deficiency, excess and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"84b0f62b2dae6835fa26902be87625c003c6c707492d3007e8f9d15420669008","revision_id":"d7e35b8b-3f77-56d9-90b5-5f542c63f321","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}