{"id":"2575366b-c085-5cf7-862b-017b1d6a54c1","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-a549-trxr","predicate":"reduces","statement":"Myricetin above 50 micromolar reduced thioredoxin reductase activity in A549-cell lysates.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fdf97163-5122-594f-92bd-fc967b609a3e","mechanism_event_label":"The cell experiment required a different concentration range.","subject":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"object":{"id":"5cfcf8bb-1d8c-5ea9-91d3-ca3b36ed4d4a","slug":"human-a549-trxr-activity","display_name":"Human A549 thioredoxin reductase activity; isoform unresolved","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"fdf97163-5122-594f-92bd-fc967b609a3e","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-a549-trxr-event","event_type":"observed_relationship","label":"The cell experiment required a different concentration range.","description":"Myricetin above 50 micromolar reduced thioredoxin reductase activity in A549-cell lysates.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5cfcf8bb-1d8c-5ea9-91d3-ca3b36ed4d4a","slug":"human-a549-trxr-activity","display_name":"Human A549 thioredoxin reductase activity; isoform unresolved","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human lung cancer cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not substitute the purified-enzyme IC50 for a cellular or clinical dose.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Myricetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The cell experiment required a different concentration range.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibition of Mammalian thioredoxin reductase by some flavonoids: implications for myricetin and quercetin anticancer activity. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16618767/ · DOI 10.1158/0008-5472.CAN-05-3310","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5f05d1d9-e0c8-5f9c-83e0-6ae4af1639c6","evidence_kind":"source_excerpt","locator":"Lines 276-282","start_line":276,"end_line":282,"excerpt":"## myricetin-a549-trxr\nThe cell experiment required a different concentration range.\nMyricetin above 50 micromolar reduced thioredoxin reductase activity in A549-cell lysates.\nModel: Human lung cancer cells.\nLimitations: Do not substitute the purified-enzyme IC50 for a cellular or clinical dose.\nEvidence access: Primary abstract\nInhibition of Mammalian thioredoxin reductase by some flavonoids: implications for myricetin and quercetin anticancer activity. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16618767/ · DOI 10.1158/0008-5472.CAN-05-3310","model_system":"Human lung cancer cells.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"58045cd9-3b27-5469-bb4b-e1c6a9bdca34","stable_key":"import-8182578e-8d21-598e-a3cb-e62d480d2251","title":"Myricetin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"0abf512275cf3aae9e4ec8544f1cde97e0155d0cfb5be12a193cdd5a898386a7","revision_id":"422e342f-a55c-5e73-ba98-43df560f49b9","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}