{"id":"255d939a-28ba-5944-8f3e-254d0ba98a07","stable_key":"c953cca8-774f-596d-b27a-f13c2c0b5fce:dhc-glucuronidation-in-gut","predicate":"is_metabolised_by","statement":"The structure of the glucuronide metabolites of capsaicin and dihydrocapsaicin that appeared in the perfusate during 90 minutes of luminal perfusion of a standardised Capsicum extract was identified by mass spectrometry.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"b4df915f-c166-57a3-af75-1712d313c951","mechanism_event_label":"The gut also attaches a sugar group to both compounds while they are being absorbed.","subject":{"id":"6259a8e0-9611-5f60-9e1b-53bbd8eb1afd","slug":"dihydrocapsaicin","display_name":"Dihydrocapsaicin","entity_type_key":"small_molecule"},"object":{"id":"16730ea4-47d4-547c-b0d0-384e94c37286","slug":"capsaicinoid-glucuronide","display_name":"Glucuronide conjugates of capsaicin and dihydrocapsaicin","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"b4df915f-c166-57a3-af75-1712d313c951","stable_key":"c953cca8-774f-596d-b27a-f13c2c0b5fce:dhc-glucuronidation-in-gut-event","event_type":"observed_intervention","label":"The gut also attaches a sugar group to both compounds while they are being absorbed.","description":"The structure of the glucuronide metabolites of capsaicin and dihydrocapsaicin that appeared in the perfusate during 90 minutes of luminal perfusion of a standardised Capsicum extract was identified by mass spectrometry.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9cdd3a64-5185-5998-82a6-d25c8409b998","slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"},"role":"co_conjugated_compound","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a90f9522-4936-5f31-b64e-f11a9c3d9e06","slug":"first-pass-metabolism","display_name":"First-pass metabolism during and after absorption","entity_type_key":"cellular_process"},"role":"process","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"6259a8e0-9611-5f60-9e1b-53bbd8eb1afd","slug":"dihydrocapsaicin","display_name":"Dihydrocapsaicin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"16730ea4-47d4-547c-b0d0-384e94c37286","slug":"capsaicinoid-glucuronide","display_name":"Glucuronide conjugates of capsaicin and dihydrocapsaicin","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/dihydrocapsaicin-research/25462121.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"667a4d33ee4b6423efe4532d0138ba6eb68a08fcc41b33ff5d04f442ad6f6177\", \"start_char\": 0, \"end_char\": 1576, \"text_sha256\": \"667a4d33ee4b6423efe4532d0138ba6eb68a08fcc41b33ff5d04f442ad6f6177\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Validated reverse-phase HPLC with fluorescence detection applied to rat small intestine luminal perfusion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Standardised Capsicum extract at 30 micrograms per millilitre perfused luminally for 90 minutes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Primarily a method development paper. The substantive finding recorded here is the identification of glucuronide conjugates of both compounds in the perfusate by mass spectrometry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Dihydrocapsaicin research collection; topical membership is not evidence of a direct clinical effect, and dihydrocapsaicin is recorded separately from capsaicin.","comparator":null,"unit":null,"notes":"","entity":{"slug":"dihydrocapsaicin","display_name":"Dihydrocapsaicin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rat","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The gut also attaches a sugar group to both compounds while they are being absorbed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[dhc-p25462121] A validated HPLC-FLD method for analysis of intestinal absorption and metabolism of capsaicin and dihydrocapsaicin in the rat. (2015). https://pubmed.ncbi.nlm.nih.gov/25462121/ DOI: 10.1016/j.jpba.2014.10.007","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Small intestine lumen","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c6513716-b2e5-5aa3-86cd-2c6b60060255","evidence_kind":"source_excerpt","locator":"Lines 556-567","start_line":556,"end_line":567,"excerpt":"### dhc-glucuronidation-in-gut\nThe structure of the glucuronide metabolites of capsaicin and dihydrocapsaicin that appeared in the perfusate during 90 minutes of luminal perfusion of a standardised Capsicum extract was identified by mass spectrometry.\nCondition category: normal\nnutrient_topic: Dihydrocapsaicin research collection; topical membership is not evidence of a direct clinical effect, and dihydrocapsaicin is recorded separately from capsaicin.\nplain_language: The gut also attaches a sugar group to both compounds while they are being absorbed.\norganism: Rat\ntissue_or_cell_type: Small intestine lumen\nexperimental_model: Validated reverse-phase HPLC with fluorescence detection applied to rat small intestine luminal perfusion\nlimitations: Primarily a method development paper. The substantive finding recorded here is the identification of glucuronide conjugates of both compounds in the perfusate by mass spectrometry.\nexposure: Standardised Capsicum extract at 30 micrograms per millilitre perfused luminally for 90 minutes\nevidence_span: {\"source_cache\": \"artifacts/dihydrocapsaicin-research/25462121.abstract.txt\", \"locator\": \"Indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"667a4d33ee4b6423efe4532d0138ba6eb68a08fcc41b33ff5d04f442ad6f6177\", \"start_char\": 0, \"end_char\": 1576, \"text_sha256\": \"667a4d33ee4b6423efe4532d0138ba6eb68a08fcc41b33ff5d04f442ad6f6177\"}\n[dhc-p25462121] A validated HPLC-FLD method for analysis of intestinal absorption and metabolism of capsaicin and dihydrocapsaicin in the rat. (2015). https://pubmed.ncbi.nlm.nih.gov/25462121/ DOI: 10.1016/j.jpba.2014.10.007","model_system":"Validated reverse-phase HPLC with fluorescence detection applied to rat small intestine luminal perfusion","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [dhc-p25462121] A validated HPLC-FLD method for analysis of intestinal absorption and metabolism of capsaicin and dihydrocapsaicin in the rat. 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