{"id":"253fb3fa-7b0e-5a8c-8f72-cfd33a557050","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:pep-e4p-dahp","predicate":"converted_to_in_recorded_reaction","statement":"DAHP synthase combines PEP and erythrose-4-phosphate into DAHP.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"f7fced45-f963-5fee-8001-cc027e23f20d","mechanism_event_label":"DAHP synthase combines PEP and erythrose-4-phosphate into DAHP.","subject":{"id":"20e5852c-38a1-54be-b486-d3d8083d9c2e","slug":"phosphoenolpyruvate","display_name":"Phosphoenolpyruvate / PEP","entity_type_key":"small_molecule"},"object":{"id":"c5dfee12-8607-526f-a956-15dadeee0e82","slug":"dahp","display_name":"3-Deoxy-D-arabino-heptulosonate 7-phosphate / DAHP","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"f7fced45-f963-5fee-8001-cc027e23f20d","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:pep-e4p-dahp-event","event_type":"biochemical_reaction","label":"DAHP synthase combines PEP and erythrose-4-phosphate into DAHP.","description":"**Carbon entry: DAHP formation.** The E. coli pathway map joins phosphoenolpyruvate (PEP) and erythrose-4-phosphate (E4P) through DAHP synthase to form 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP), releasing phosphate. These inputs connect central carbon metabolism to aromatic biosynthesis. The cited engineering study provides pathway and flux-redistribution context; its diagram is not a new kinetic assay for every reaction. Its 2020 publisher correction replaces a duplicated Figure 7 and corrects its legend; the current corrected article is the reference. [Metabolic engineering of Escherichia coli for shikimate pathway derivative production from glucose-xylose co-substrate.](https://pubmed.ncbi.nlm.nih.gov/31937786/) [Publisher correction to metabolic engineering of Escherichia coli for shikimate pathway derivative production](https://www.nature.com/articles/s41467-020-14710-5)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"20e5852c-38a1-54be-b486-d3d8083d9c2e","slug":"phosphoenolpyruvate","display_name":"Phosphoenolpyruvate / PEP","entity_type_key":"small_molecule"},"role":"input","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c5dfee12-8607-526f-a956-15dadeee0e82","slug":"dahp","display_name":"3-Deoxy-D-arabino-heptulosonate 7-phosphate / DAHP","entity_type_key":"small_molecule"},"role":"output","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ef8fb66f-e1cd-5efc-91e0-63dccde4ab0b","slug":"ecoli-dahp-synthases","display_name":"Escherichia coli DAHP synthase isoenzymes","entity_type_key":"protein"},"role":"catalyst","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"f5a6a9d9-844c-5624-b2a1-d2fd17b7076e","slug":"erythrose-4-phosphate","display_name":"D-Erythrose 4-phosphate / E4P","entity_type_key":"small_molecule"},"role":"input","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"b28dfc54-a5ef-5f9e-ac1f-890b68e58dc3","slug":"inorganic-phosphate","display_name":"Inorganic phosphate","entity_type_key":"chemical_species"},"role":"output","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text retrieved; relevant methods/results/figures reviewed. Selective extraction, not raw-data reanalysis or exhaustive supplemental extraction.\nPrimary correction read: duplicated Fig. 7 and incorrect panel reference corrected; not a retraction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Escherichia coli biochemical pathway; source-specific enzyme evidence recorded in the passage.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"DAHP synthase combines PEP and erythrose-4-phosphate into DAHP.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Metabolic engineering of Escherichia coli for shikimate pathway derivative production from glucose-xylose co-substrate. | 2020 | DOI 10.1038/s41467-019-14024-1 | PMID 31937786 | https://pubmed.ncbi.nlm.nih.gov/31937786/ | https://doi.org/10.1038/s41467-019-14024-1 | https://pmc.ncbi.nlm.nih.gov/articles/PMC6959354/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Publisher correction to metabolic engineering of Escherichia coli for shikimate pathway derivative production | 2020 | DOI 10.1038/s41467-020-14710-5 | https://www.nature.com/articles/s41467-020-14710-5","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 9-9; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c58113bb-4e39-541b-8f26-06168c892507","evidence_kind":"source_excerpt","locator":"Lines 9-9","start_line":9,"end_line":9,"excerpt":"**Carbon entry: DAHP formation.** The E. coli pathway map joins phosphoenolpyruvate (PEP) and erythrose-4-phosphate (E4P) through DAHP synthase to form 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP), releasing phosphate. These inputs connect central carbon metabolism to aromatic biosynthesis. The cited engineering study provides pathway and flux-redistribution context; its diagram is not a new kinetic assay for every reaction. Its 2020 publisher correction replaces a duplicated Figure 7 and corrects its legend; the current corrected article is the reference. [Metabolic engineering of Escherichia coli for shikimate pathway derivative production from glucose-xylose co-substrate.](https://pubmed.ncbi.nlm.nih.gov/31937786/) [Publisher correction to metabolic engineering of Escherichia coli for shikimate pathway derivative production](https://www.nature.com/articles/s41467-020-14710-5)","model_system":"Escherichia coli biochemical pathway; source-specific enzyme evidence recorded in the passage.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. 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