{"id":"25379271-cf53-5545-8b28-af897c8354a8","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-trans-pendrin-efflux","predicate":"mediates_efflux","statement":"Human pendrin expression supported iodide efflux in COS-7 and CHO mammalian-cell transport experiments.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"7964dd0a-aa38-5c94-abbb-c755fce85e25","mechanism_event_label":"Pendrin can let iodide leave cells.","subject":{"id":"57fef31d-e7e6-5b6f-aee1-77fb94592ce7","slug":"slc26a4","display_name":"Human pendrin / SLC26A4","entity_type_key":"protein"},"object":{"id":"99611171-98f5-5310-af88-a988e8f0e6a6","slug":"cellular-iodide-efflux","display_name":"Cellular iodide efflux","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7964dd0a-aa38-5c94-abbb-c755fce85e25","stable_key":"aaa7baba-8689-56ab-ba1e-b71542bcb8e9:iodine-trans-pendrin-efflux-event","event_type":"biochemical_relationship","label":"Pendrin can let iodide leave cells.","description":"Human pendrin expression supported iodide efflux in COS-7 and CHO mammalian-cell transport experiments.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"83b11ca6-52c7-5a1b-8c39-d9cdf89244e3","slug":"iodide","display_name":"Iodide ion","entity_type_key":"ion"},"role":"exported_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"0d051c22-c0d2-5c22-91ad-4ee6894dc7a0","slug":"slc5a5","display_name":"Human sodium/iodide symporter / SLC5A5","entity_type_key":"protein"},"role":"loading_transporter","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"57fef31d-e7e6-5b6f-aee1-77fb94592ce7","slug":"slc26a4","display_name":"Human pendrin / SLC26A4","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"99611171-98f5-5310-af88-a988e8f0e6a6","slug":"cellular-iodide-efflux","display_name":"Cellular iodide efflux","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human pendrin expressed in COS-7 and CHO cells with NIS; rat FRTL-5 comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Pendrin/NIS transfection; concentrations not provided in abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression-cell data do not establish exclusive thyroid efflux control or transport specificity in every tissue.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Iodine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"iodine","display_name":"Iodine","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human protein in monkey COS-7 and hamster CHO cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Pendrin can let iodide leave cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[iodine-trans-pendrin-efflux2002] Pendrin is an iodide-specific apical porter responsible for iodide efflux from thyroid cells. (2002). https://pubmed.ncbi.nlm.nih.gov/12107249/ DOI: 10.1210/jcem.87.7.8679","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cultured-cell plasma membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f0ac939b-2e8f-5bed-8d0e-c4a96585ca31","evidence_kind":"source_excerpt","locator":"Lines 297-308","start_line":297,"end_line":308,"excerpt":"### iodine-trans-pendrin-efflux\nHuman pendrin expression supported iodide efflux in COS-7 and CHO mammalian-cell transport experiments.\nCondition category: normal\nnutrient_topic: Iodine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Pendrin can let iodide leave cells.\norganism: Human protein in monkey COS-7 and hamster CHO cells\ntissue_or_cell_type: Cultured-cell plasma membrane\nexperimental_model: Human pendrin expressed in COS-7 and CHO cells with NIS; rat FRTL-5 comparison\nlimitations: Expression-cell data do not establish exclusive thyroid efflux control or transport specificity in every tissue.\nexposure: Pendrin/NIS transfection; concentrations not provided in abstract.\ncross_nutrient: false\n[iodine-trans-pendrin-efflux2002] Pendrin is an iodide-specific apical porter responsible for iodide efflux from thyroid cells. (2002). https://pubmed.ncbi.nlm.nih.gov/12107249/ DOI: 10.1210/jcem.87.7.8679","model_system":"Human pendrin expressed in COS-7 and CHO cells with NIS; rat FRTL-5 comparison","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [iodine-trans-pendrin-efflux2002] Pendrin is an iodide-specific apical porter responsible for iodide efflux from thyroid cells. (2002). https://pubmed.ncbi.nlm.nih.gov/12107249/ DOI: 10.1210/jcem.87.7.8679","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"76da623d-a34a-5a5c-a942-d7571a85c486","stable_key":"import-aaa7baba-8689-56ab-ba1e-b71542bcb8e9","title":"Iodine: thyroid hormone production, deficiency, excess and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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