{"id":"253519be-53c3-5c23-ab31-042ebec10ae8","stable_key":"33bef151-bdc7-5892-aff6-0345ab149739:natto-carries-the-fibrinolytic-enzyme","predicate":"contains","statement":"A strong fibrinolytic activity averaging about 40 CU, in plasmin units, per gram wet weight was demonstrated in natto, and the enzyme responsible was extracted with saline and named nattokinase.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"fc702131-10ab-540f-8d42-8ee12541728d","mechanism_event_label":"A strong fibrinolytic activity averaging about 40 CU, in plasmin units, per gram wet weight was demonstrated in natto, and the enzyme responsible was extracted with saline and named nattokinase.","subject":{"id":"b7d0177d-170d-5ea4-995f-eec6760f640e","slug":"natto","display_name":"Natto, fermented soybean food","entity_type_key":"botanical"},"object":{"id":"6b03a125-209a-5060-8b35-ec9e0e54033c","slug":"nattokinase","display_name":"Nattokinase / subtilisin NAT","entity_type_key":"peptide"},"evidence_count":1,"mechanism_event":{"id":"fc702131-10ab-540f-8d42-8ee12541728d","stable_key":"33bef151-bdc7-5892-aff6-0345ab149739:natto-carries-the-fibrinolytic-enzyme-event","event_type":"biochemical_relationship","label":"A strong fibrinolytic activity averaging about 40 CU, in plasmin units, per gram wet weight was demonstrated in natto, and the enzyme responsible was extracted with saline and named nattokinase.","description":"Saline extract of the food","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b7d0177d-170d-5ea4-995f-eec6760f640e","slug":"natto","display_name":"Natto, fermented soybean food","entity_type_key":"botanical"},"role":"acting component","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b03a125-209a-5060-8b35-ec9e0e54033c","slug":"nattokinase","display_name":"Nattokinase / subtilisin NAT","entity_type_key":"peptide"},"role":"component the finding is about","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"duration","value_text":"Not stated here","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Natto, fermented soybean food","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Not applicable","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The discovery report. Its estimates of molecular weight, about 20,000, and isoelectric point, 8.6, predate the sequence and are superseded by the 275-residue chain of 27,728 Da. Activity is given in CU, plasmin units, which is a different unit system from the FU used by every product in this chapter and is not convertible to it. Diisopropyl fluorophosphate strongly inhibited the activity, consistent with the serine-protease assignment made later.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Natto, fermented soybean food","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A strong fibrinolytic activity averaging about 40 CU, in plasmin units, per gram wet weight was demonstrated in natto, and the enzyme responsible was extracted with saline and named nattokinase.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A novel fibrinolytic enzyme (nattokinase) in the vegetable cheese Natto; a typical and popular soybean food in the Japanese diet. (1987) https://pubmed.ncbi.nlm.nih.gov/3478223/ DOI: 10.1007/BF01956052","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Saline extract of the food","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"bd631f83-918b-57f2-aabf-94e3dfaedce3","evidence_kind":"source_excerpt","locator":"Lines 26-26","start_line":26,"end_line":26,"excerpt":"A strong fibrinolytic activity averaging about 40 CU, in plasmin units, per gram wet weight was demonstrated in natto, and the enzyme responsible was extracted with saline and named nattokinase.","model_system":"Natto, fermented soybean food","directness":"reported_statement","verification_status":"source_derived_draft","notes":"","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"41cb127c-1c23-53aa-b7d0-5ec7f6fa3c99","stable_key":"import-33bef151-bdc7-5892-aff6-0345ab149739","title":"Nattokinase: what the purified enzyme cleaves, what survives being eaten, and the gap between the two (2026-09-23)","document_type":"imported_text","citation_label":"Original AI-assisted curation built from a supplied entity-first document of 105 entities and 129 claims. Every reference in that document was resolved against live PubMed with its abstract read and its DOI cross-checked on 2026-09-23, and the EFSA novel-food opinion was retrieved and read in full. That check corrected two PMIDs that pointed at unrelated papers, two DOIs, and two papers recorded as carrying no erratum that do carry one; it also reversed three findings the supplied document had stated backwards. Two papers carry a published correction, recorded as such and not as a retraction. Three sources are not indexed in PubMed and are cited by what they have. Laboratory lineages are recorded, so the four papers from one group, the three from another and the two readings of a single applicant dossier cannot be counted as separate lines of support. Study-specific doses, units, populations and limitations retained; activity units are never converted between systems. Not publisher full text.","file_path":"","sha256":"34fc46e0e849d06bfa13cb099a79b50679dd6fd0de8a5a0575326afdde3259c1","revision_id":"efd063e6-e982-5f6e-a4b8-6f2ffe4185ce","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}