{"id":"252c2cb5-ed8a-5559-a8c1-106bb452b121","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-trans-znt2-h54r-coexpression","predicate":"does_not_abrogate","statement":"Coexpressing human ZnT2 H54R with wild-type ZnT2 did not abolish the increased zinc secretion produced by wild-type ZnT2 overexpression alone.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"ca609552-fea3-572b-a01f-9860ff991e58","mechanism_event_label":"In this assay, the H54R variant did not block the working ZnT2 protein.","subject":{"id":"8a4003c4-10fd-5113-ba8e-26fc273d4f3b","slug":"slc30a2-h54r","display_name":"Human ZnT2 H54R","entity_type_key":"protein_state"},"object":{"id":"515129c8-6c3c-5090-9b50-1b38d5d192fd","slug":"cellular-zinc-secretion","display_name":"Cellular zinc secretion","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ca609552-fea3-572b-a01f-9860ff991e58","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zinc-trans-znt2-h54r-coexpression-event","event_type":"biochemical_relationship","label":"In this assay, the H54R variant did not block the working ZnT2 protein.","description":"Coexpressing human ZnT2 H54R with wild-type ZnT2 did not abolish the increased zinc secretion produced by wild-type ZnT2 overexpression alone.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a527043c-0551-5ad5-8e90-033a73b8723a","slug":"slc30a2","display_name":"Human ZnT2 (SLC30A2)","entity_type_key":"protein"},"role":"coexpressed_wild_type","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"secreted_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8a4003c4-10fd-5113-ba8e-26fc273d4f3b","slug":"slc30a2-h54r","display_name":"Human ZnT2 H54R","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"515129c8-6c3c-5090-9b50-1b38d5d192fd","slug":"cellular-zinc-secretion","display_name":"Cellular zinc secretion","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Wild-type/mutant coexpression in HEK293 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"H54R plus wild type compared with wild type alone.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Negative result in an overexpression assay; it does not establish complete compensation in lactating women or the penetrance of the variant.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zinc research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"In this assay, the H54R variant did not block the working ZnT2 protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zinc-trans-17065149] Identification of a mutation in SLC30A2 (ZnT-2) in women with low milk zinc concentration that results in transient neonatal zinc deficiency. (2006). https://pubmed.ncbi.nlm.nih.gov/17065149/ DOI: 10.1074/jbc.m605821200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HEK293 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"ec8533a9-45a0-5c7b-9faf-bafdc65b3514","evidence_kind":"source_excerpt","locator":"Lines 323-334","start_line":323,"end_line":334,"excerpt":"### zinc-trans-znt2-h54r-coexpression\nCoexpressing human ZnT2 H54R with wild-type ZnT2 did not abolish the increased zinc secretion produced by wild-type ZnT2 overexpression alone.\nCondition category: machinery_impairment\nnutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: In this assay, the H54R variant did not block the working ZnT2 protein.\norganism: Homo sapiens\ntissue_or_cell_type: HEK293 cells\nexperimental_model: Wild-type/mutant coexpression in HEK293 cells\nlimitations: Negative result in an overexpression assay; it does not establish complete compensation in lactating women or the penetrance of the variant.\nexposure: H54R plus wild type compared with wild type alone.\ncross_nutrient: false\n[zinc-trans-17065149] Identification of a mutation in SLC30A2 (ZnT-2) in women with low milk zinc concentration that results in transient neonatal zinc deficiency. (2006). https://pubmed.ncbi.nlm.nih.gov/17065149/ DOI: 10.1074/jbc.m605821200","model_system":"Wild-type/mutant coexpression in HEK293 cells","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zinc-trans-17065149] Identification of a mutation in SLC30A2 (ZnT-2) in women with low milk zinc concentration that results in transient neonatal zinc deficiency. (2006). https://pubmed.ncbi.nlm.nih.gov/17065149/ DOI: 10.1074/jbc.m605821200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c5ee0fee-ce5c-58de-905a-10fb0ea0723c","stable_key":"import-6d38d43e-01e4-5641-93be-65654271e242","title":"Zinc: transport, enzyme loading, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"2e731dd54477ec1254e97df3323e1208d38f1375effed19252e71ab4f600d13a","revision_id":"c72258b9-ac09-5408-9d44-a921ad1f96a3","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}