{"id":"25288db2-9863-5009-abba-ab42f71cc49f","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-restriction-lowers-aqp2-ser256","predicate":"restriction-decreases","statement":"Dietary potassium restriction reduced Ser256-phosphorylated AQP2 in cortical and medullary collecting ducts of both male and female mice.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"12a7cbda-52d3-586d-807c-8c3d1e362f80","mechanism_event_label":"The channel activation/trafficking state changed even where total protein loss was less marked.","subject":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"object":{"id":"9f5e6aba-df3b-5051-9182-408ea99d459c","slug":"aqp2-ser256-phosphorylated","display_name":"Ser256-phosphorylated AQP2","entity_type_key":"protein_state"},"evidence_count":1,"mechanism_event":{"id":"12a7cbda-52d3-586d-807c-8c3d1e362f80","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-restriction-lowers-aqp2-ser256-event","event_type":"biochemical_relationship","label":"The channel activation/trafficking state changed even where total protein loss was less marked.","description":"Dietary potassium restriction reduced Ser256-phosphorylated AQP2 in cortical and medullary collecting ducts of both male and female mice.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"87435e1d-e1cd-57da-984b-203b2f5f29a4","slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"},"role":"upstream","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9f5e6aba-df3b-5051-9182-408ea99d459c","slug":"aqp2-ser256-phosphorylated","display_name":"Ser256-phosphorylated AQP2","entity_type_key":"protein_state"},"role":"downstream","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0cf1432e-2f5f-542c-8980-6894da46cce6","slug":"aqp2","display_name":"Aquaporin-2 / AQP2","entity_type_key":"protein"},"role":"parent-protein","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"d31225b7-8e94-5857-9ea1-67ac794b1f15","slug":"water","display_name":"H2O","entity_type_key":"small_molecule"},"role":"transported-substrate","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"endpoint","value_text":"Dietary potassium restriction reduced Ser256-phosphorylated AQP2 in cortical and medullary collecting ducts of both male and female mice.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental-exposure","value_text":"Male and female C57BL/6J mice exposed to graded potassium restriction for eight days; water-balance and collecting-duct signaling measurements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Male and female C57BL/6J mice exposed to graded potassium restriction for eight days; water-balance and collecting-duct signaling measurements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Sex and segment influenced severity; this does not establish a universal human plasma-potassium threshold or a unique responsible kinase.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The channel activation/trafficking state changed even where total protein loss was less marked.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[al-qusairi-2021-vasopressin] Rapid development of vasopressin resistance in dietary K+ deficiency (2021). https://pubmed.ncbi.nlm.nih.gov/33749322/ DOI: 10.1152/ajprenal.00655.2020","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"cortical and medullary collecting duct","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"f429aa41-ab67-5969-8fa4-1bbe7b022915","evidence_kind":"source_excerpt","locator":"Lines 1412-1423","start_line":1412,"end_line":1423,"excerpt":"### k-restriction-lowers-aqp2-ser256\nDietary potassium restriction reduced Ser256-phosphorylated AQP2 in cortical and medullary collecting ducts of both male and female mice.\nCondition category: nutrient_deficiency\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The channel activation/trafficking state changed even where total protein loss was less marked.\norganism: Mus musculus\ntissue_or_cell_type: cortical and medullary collecting duct\nexperimental_model: Male and female C57BL/6J mice exposed to graded potassium restriction for eight days; water-balance and collecting-duct signaling measurements.\nlimitations: Sex and segment influenced severity; this does not establish a universal human plasma-potassium threshold or a unique responsible kinase.\nexperimental-exposure: Male and female C57BL/6J mice exposed to graded potassium restriction for eight days; water-balance and collecting-duct signaling measurements.\nendpoint: Dietary potassium restriction reduced Ser256-phosphorylated AQP2 in cortical and medullary collecting ducts of both male and female mice.\n[al-qusairi-2021-vasopressin] Rapid development of vasopressin resistance in dietary K+ deficiency (2021). https://pubmed.ncbi.nlm.nih.gov/33749322/ DOI: 10.1152/ajprenal.00655.2020","model_system":"Male and female C57BL/6J mice exposed to graded potassium restriction for eight days; water-balance and collecting-duct signaling measurements.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [al-qusairi-2021-vasopressin] Rapid development of vasopressin resistance in dietary K+ deficiency (2021). https://pubmed.ncbi.nlm.nih.gov/33749322/ DOI: 10.1152/ajprenal.00655.2020","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}