{"id":"24ceb8e6-c80c-5c75-bf02-240687f77a20","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:tyms-dump-methylation","predicate":"converts","statement":"Human TYMS uses 5,10-methylene-THF to convert dUMP to dTMP, producing dihydrofolate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"62533860-fa91-5bab-b187-af7195f3857f","mechanism_event_label":"Folate supplies the carbon and reducing power to make a DNA base.","subject":{"id":"4787b939-16f1-56d3-8a66-acc5745625dd","slug":"tyms","display_name":"Human thymidylate synthase / TYMS","entity_type_key":"protein"},"object":{"id":"40631c80-c592-5f1e-89b3-fe8910f95b2b","slug":"dump","display_name":"Deoxyuridine monophosphate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"62533860-fa91-5bab-b187-af7195f3857f","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:tyms-dump-methylation-event","event_type":"biochemical_relationship","label":"Folate supplies the carbon and reducing power to make a DNA base.","description":"Human TYMS uses 5,10-methylene-THF to convert dUMP to dTMP, producing dihydrofolate.","status":"provisional","compartment":{"slug":"nucleus","display_name":"Nucleus"},"participants":[{"entity":{"id":"0413b195-325e-5757-a72b-f445d65c17bc","slug":"5-10-methylenetetrahydrofolate","display_name":"5,10-Methylenetetrahydrofolate","entity_type_key":"small_molecule"},"role":"carbon and hydride donor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8d6977b6-1591-5a49-89ff-3871c328b4ad","slug":"dtmp","display_name":"Deoxythymidine monophosphate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b6feb26d-6f4d-5232-b54a-650e125fcdf8","slug":"dihydrofolate","display_name":"7,8-Dihydrofolate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4787b939-16f1-56d3-8a66-acc5745625dd","slug":"tyms","display_name":"Human thymidylate synthase / TYMS","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"40631c80-c592-5f1e-89b3-fe8910f95b2b","slug":"dump","display_name":"Deoxyuridine monophosphate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Purified human TYMS reaction assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Assay conditions described in the linked primary study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Purified-enzyme chemistry does not specify cellular flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Folate supplies the carbon and reducing power to make a DNA base.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[islam-2018] Bacterial versus human thymidylate synthase: Kinetics and functionality (2018). https://pubmed.ncbi.nlm.nih.gov/29715278/ DOI: 10.1371/journal.pone.0196506","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cell-free","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"ce752bbb-c299-5a7c-96d4-c0e058afa7ec","evidence_kind":"source_excerpt","locator":"Lines 973-983","start_line":973,"end_line":983,"excerpt":"### tyms-dump-methylation\nHuman TYMS uses 5,10-methylene-THF to convert dUMP to dTMP, producing dihydrofolate.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Folate supplies the carbon and reducing power to make a DNA base.\norganism: Homo sapiens\ntissue_or_cell_type: Cell-free\nexperimental_model: Purified human TYMS reaction assays\nlimitations: Purified-enzyme chemistry does not specify cellular flux.\nexposure: Assay conditions described in the linked primary study.\n[islam-2018] Bacterial versus human thymidylate synthase: Kinetics and functionality (2018). https://pubmed.ncbi.nlm.nih.gov/29715278/ DOI: 10.1371/journal.pone.0196506","model_system":"Purified human TYMS reaction assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [islam-2018] Bacterial versus human thymidylate synthase: Kinetics and functionality (2018). https://pubmed.ncbi.nlm.nih.gov/29715278/ DOI: 10.1371/journal.pone.0196506","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}