{"id":"24b2919d-7092-5396-b62f-8064c20a4e24","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:ydib-specificity","predicate":"reported_relationship","statement":"E. coli YdiB accepts NAD/NADP cofactors for quinate/shikimate dehydrogenase activity.","claim_class":"mechanistic","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"0359b6c0-9954-5774-91bf-f1de8a84bdd9","mechanism_event_label":"E. coli YdiB accepts NAD/NADP cofactors for quinate/shikimate dehydrogenase activity.","subject":{"id":"96032674-7990-585d-9441-0806cdfee84d","slug":"ecoli-ydib","display_name":"Escherichia coli quinate/shikimate dehydrogenase / YdiB","entity_type_key":"protein"},"object":{"id":"be6e4670-953f-5a4e-97da-1e536a54e0e4","slug":"nadp-plus","display_name":"NADP+","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"0359b6c0-9954-5774-91bf-f1de8a84bdd9","stable_key":"31b1baa4-4113-5541-b9e7-fe44a5253a07:ydib-specificity-event","event_type":"biochemical_relationship","label":"E. coli YdiB accepts NAD/NADP cofactors for quinate/shikimate dehydrogenase activity.","description":"**NADPH supplies reducing equivalents.** E. coli AroE reduces DHS to shikimate using NADPH, producing NADP+. 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A common structural framework for different activities.](https://pubmed.ncbi.nlm.nih.gov/12637497/) [Metabolic engineering of Escherichia coli for shikimate pathway derivative production from glucose-xylose co-substrate.](https://pubmed.ncbi.nlm.nih.gov/31937786/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"96032674-7990-585d-9441-0806cdfee84d","slug":"ecoli-ydib","display_name":"Escherichia coli quinate/shikimate dehydrogenase / YdiB","entity_type_key":"protein"},"role":"tested factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"be6e4670-953f-5a4e-97da-1e536a54e0e4","slug":"nadp-plus","display_name":"NADP+","entity_type_key":"small_molecule"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary indexed abstract reviewed; full results, tables and supplements not independently extracted.\nPrimary full text retrieved; relevant methods/results/figures reviewed. 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