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(2017). https://pubmed.ncbi.nlm.nih.gov/28052864/ DOI: 10.1152/ajpcell.00300.2016","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Colonic epithelial NCM460 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"4e3d7ae9-2570-557f-88f3-317fa4a98466","evidence_kind":"source_excerpt","locator":"Lines 1118-1129","start_line":1118,"end_line":1129,"excerpt":"### b7-lps-uptake\nLPS reduced carrier-mediated biotin uptake in NCM460 cells; CK2 inhibition and a putative CK2-site SMVT mutant supported a CK2-related trafficking mechanism.\nCondition category: machinery_impairment\nnutrient_topic: Biotin research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Inflammatory exposure interfered with biotin entry in this model.\norganism: Homo sapiens\ntissue_or_cell_type: Colonic epithelial NCM460 cells\nexperimental_model: LPS exposure of human NCM460 cells with separate mouse experiments\nlimitations: CK2 involvement is supported experimentally but the full causal sequence remains qualified; whole-body human deficiency was not measured.\nexposure: LPS, CK2 inhibitors and SMVT Thr78Ala mutation\nevidence_span: {\"source_cache\": \"artifacts/biotin-research/28052864.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"2f27f27a7730837eff6529a7eccf67b3587df28af01ac0507d1b692410483703\", \"start_char\": 0, \"end_char\": 1549, \"text_sha256\": \"2f27f27a7730837eff6529a7eccf67b3587df28af01ac0507d1b692410483703\"}\n[b7-p28052864] Lipopolysaccharide inhibits colonic biotin uptake via interference with membrane expression of its transporter: a role for a casein kinase 2-mediated pathway. (2017). https://pubmed.ncbi.nlm.nih.gov/28052864/ DOI: 10.1152/ajpcell.00300.2016","model_system":"LPS exposure of human NCM460 cells with separate mouse experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b7-p28052864] Lipopolysaccharide inhibits colonic biotin uptake via interference with membrane expression of its transporter: a role for a casein kinase 2-mediated pathway. 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