{"id":"249c0901-390c-5a52-8fe2-8e5495525fbf","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-aars-alanine-binding","predicate":"binds","statement":"Binding assays with recombinant human AARS1 measured alanine binding with a reported dissociation constant of 0.45 micromolar, compared with 2.06 micromolar for lactate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2b944c67-8404-5a27-b1cd-71aaed719836","mechanism_event_label":"One enzyme can recognize alanine and a second metabolite.","subject":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"object":{"id":"cc0bb2ab-8cf9-54a7-af05-3681ef835010","slug":"aars1","display_name":"Human cytosolic alanyl-tRNA synthetase / AARS1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"2b944c67-8404-5a27-b1cd-71aaed719836","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-aars-alanine-binding-event","event_type":"observed_relationship","label":"One enzyme can recognize alanine and a second metabolite.","description":"Binding assays with recombinant human AARS1 measured alanine binding with a reported dissociation constant of 0.45 micromolar, compared with 2.06 micromolar for lactate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"cc0bb2ab-8cf9-54a7-af05-3681ef835010","slug":"aars1","display_name":"Human cytosolic alanyl-tRNA synthetase / AARS1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5ebd9a7c-b151-5b70-b70d-7d33067d4ebf","slug":"lactate","display_name":"L-Lactate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human AARS1 binding experiments in a gastric-cancer mechanism study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"These assay affinities do not establish intracellular competition or an effect of oral alanine on lactylation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"One enzyme can recognize alanine and a second metabolite.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The alanyl-tRNA synthetase AARS1 moonlights as a lactyltransferase to promote YAP signaling in gastric cancer. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38512451/ · DOI 10.1172/JCI174587","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"75becfc3-709f-54bc-9040-74d79f66e8e9","evidence_kind":"source_excerpt","locator":"Lines 64-70","start_line":64,"end_line":70,"excerpt":"## alanine-aars-alanine-binding\nOne enzyme can recognize alanine and a second metabolite.\nBinding assays with recombinant human AARS1 measured alanine binding with a reported dissociation constant of 0.45 micromolar, compared with 2.06 micromolar for lactate.\nModel: Purified human AARS1 binding experiments in a gastric-cancer mechanism study.\nLimitations: These assay affinities do not establish intracellular competition or an effect of oral alanine on lactylation.\nEvidence access: Primary full text\nThe alanyl-tRNA synthetase AARS1 moonlights as a lactyltransferase to promote YAP signaling in gastric cancer. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38512451/ · DOI 10.1172/JCI174587","model_system":"Purified human AARS1 binding experiments in a gastric-cancer mechanism study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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