{"id":"2456ede4-0cfb-5343-8861-8f961d6575d7","stable_key":"fe6af7fc-7372-5c4f-9c2f-2bbf56695397:phosphorus-rbc-dpg-null","predicate":"has_no_detected_concentration_dependence","statement":"No dependence of erythrocyte 2,3-DPG concentration on cellular Pi was detected in the ICU dataset.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"0aa95761-3a29-536e-b2e3-fee4e6080a53","mechanism_event_label":"A lower phosphate measurement did not automatically mean lower 2,3-DPG.","subject":{"id":"ee63fe8e-92f3-552f-8049-a5f1fa1e1523","slug":"phosphate-ion","display_name":"Inorganic phosphate (Pi; protonation depends on pH)","entity_type_key":"ion"},"object":{"id":"c8c9eb30-3221-5250-8047-3c538411d875","slug":"erythrocyte-2-3-dpg-concentration","display_name":"Human erythrocyte 2,3-DPG concentration","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"0aa95761-3a29-536e-b2e3-fee4e6080a53","stable_key":"fe6af7fc-7372-5c4f-9c2f-2bbf56695397:phosphorus-rbc-dpg-null-event","event_type":"observed_intervention","label":"A lower phosphate measurement did not automatically mean lower 2,3-DPG.","description":"No dependence of erythrocyte 2,3-DPG concentration on cellular Pi was detected in the ICU dataset.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ee63fe8e-92f3-552f-8049-a5f1fa1e1523","slug":"phosphate-ion","display_name":"Inorganic phosphate (Pi; protonation depends on pH)","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c8c9eb30-3221-5250-8047-3c538411d875","slug":"erythrocyte-2-3-dpg-concentration","display_name":"Human erythrocyte 2,3-DPG concentration","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/phosphorus-research/4042545.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"474d2b2722565fa0e4c2f0ddf4a332715ce176f9325b9af5c22027bd6ac93e43\", \"start_char\": 0, \"end_char\": 1711, \"text_sha256\": \"474d2b2722565fa0e4c2f0ddf4a332715ce176f9325b9af5c22027bd6ac93e43\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"ICU erythrocyte measurements and normal-donor cell incubation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Plasma Pi 0.1–4.2 mmol/L in patients; normal-donor cells incubated at 1 or 5 mmol/L Pi","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Observed ranges are not universal thresholds; ATP concentration, ATP synthetic flux and 2,3-DPG are separate endpoints.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Phosphorus research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"phosphorus","display_name":"Phosphorus","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A lower phosphate measurement did not automatically mean lower 2,3-DPG.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[phosphorus-p4042545] Phosphate metabolism in erythrocytes of critically ill patients. (1985). https://pubmed.ncbi.nlm.nih.gov/4042545/ DOI: 10.1042/cs0690435","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Red blood cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f8be65d7-97e5-56f7-b4a9-b2962c0d507e","evidence_kind":"source_excerpt","locator":"Lines 1076-1087","start_line":1076,"end_line":1087,"excerpt":"### phosphorus-rbc-dpg-null\nNo dependence of erythrocyte 2,3-DPG concentration on cellular Pi was detected in the ICU dataset.\nCondition category: normal\nnutrient_topic: Phosphorus research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A lower phosphate measurement did not automatically mean lower 2,3-DPG.\norganism: Human\ntissue_or_cell_type: Red blood cells\nexperimental_model: ICU erythrocyte measurements and normal-donor cell incubation\nlimitations: Observed ranges are not universal thresholds; ATP concentration, ATP synthetic flux and 2,3-DPG are separate endpoints.\nexposure: Plasma Pi 0.1–4.2 mmol/L in patients; normal-donor cells incubated at 1 or 5 mmol/L Pi\nevidence_span: {\"source_cache\": \"artifacts/phosphorus-research/4042545.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"474d2b2722565fa0e4c2f0ddf4a332715ce176f9325b9af5c22027bd6ac93e43\", \"start_char\": 0, \"end_char\": 1711, \"text_sha256\": \"474d2b2722565fa0e4c2f0ddf4a332715ce176f9325b9af5c22027bd6ac93e43\"}\n[phosphorus-p4042545] Phosphate metabolism in erythrocytes of critically ill patients. 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