{"id":"24480746-f7f5-5341-92ab-89183d25c6fc","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-adma-nos","predicate":"inhibits","statement":"In-vitro and in-vivo experiments identified ADMA as an endogenous inhibitor of NO synthesis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"847a03fe-81fc-56d1-a8cf-0196a97b1837","mechanism_event_label":"An internal inhibitor can limit the pathway even when arginine is present.","subject":{"id":"6c8e2535-0002-5b29-bb3d-36cb341b85d2","slug":"asymmetric-dimethylarginine","display_name":"Asymmetric dimethylarginine / ADMA","entity_type_key":"small_molecule"},"object":{"id":"28ad7da3-dfb0-544f-a6ea-58a375f0abe8","slug":"nos-derived-nitric-oxide-production","display_name":"NOS-dependent nitric oxide production, model specified","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"847a03fe-81fc-56d1-a8cf-0196a97b1837","stable_key":"fd37d270-3395-56d6-9f1c-ddda56e606f6:arg-adma-nos-event","event_type":"observed_relationship","label":"An internal inhibitor can limit the pathway even when arginine is present.","description":"In-vitro and in-vivo experiments identified ADMA as an endogenous inhibitor of NO synthesis.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6c8e2535-0002-5b29-bb3d-36cb341b85d2","slug":"asymmetric-dimethylarginine","display_name":"Asymmetric dimethylarginine / ADMA","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"28ad7da3-dfb0-544f-a6ea-58a375f0abe8","slug":"nos-derived-nitric-oxide-production","display_name":"NOS-dependent nitric oxide production, model specified","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"eeca3c9e-7749-5677-b7bd-37fe8f7c228d","slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Experimental NO assays and human renal-failure observations.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The abstract does not isolate a single NOS isoform.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Arginine collection; tissue, species, dose and formulation distinctions retained.","comparator":null,"unit":null,"notes":"","entity":{"slug":"arginine","display_name":"L-Arginine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An internal inhibitor can limit the pathway even when arginine is present.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Accumulation of an endogenous inhibitor of nitric oxide synthesis in chronic renal failure. · 1992 · https://pubmed.ncbi.nlm.nih.gov/1347093/ · DOI 10.1016/0140-6736(92)90865-z","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0631e125-ec44-5b14-b081-5ef7e839970b","evidence_kind":"source_excerpt","locator":"Lines 94-100","start_line":94,"end_line":100,"excerpt":"## arg-adma-nos\nAn internal inhibitor can limit the pathway even when arginine is present.\nIn-vitro and in-vivo experiments identified ADMA as an endogenous inhibitor of NO synthesis.\nModel: Experimental NO assays and human renal-failure observations.\nLimitations: The abstract does not isolate a single NOS isoform.\nEvidence access: Primary abstract\nAccumulation of an endogenous inhibitor of nitric oxide synthesis in chronic renal failure. · 1992 · https://pubmed.ncbi.nlm.nih.gov/1347093/ · DOI 10.1016/0140-6736(92)90865-z","model_system":"Experimental NO assays and human renal-failure observations.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; primary evidence access stated per claim.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"bb9a67ea-904f-5b8a-93a4-459081212bdd","stable_key":"import-fd37d270-3395-56d6-9f1c-ddda56e606f6","title":"L-Arginine: transport, metabolic branches, nutrient interactions, availability and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"ec0e9a3fb72b7485534e4372a074ac7624a399ff962e55690eb462217b5149ec","revision_id":"af027270-5535-51ae-85ff-9c76c518c962","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}