{"id":"244639f9-b74b-5451-aead-414d7f233ceb","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-placenta-il18","predicate":"reduces","statement":"Adding 1 mM ergothioneine for 24 hours reduced IL-18 production in placental explants from women with gestational diabetes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"4f264669-ed08-59a3-9a04-ca9d0101b831","mechanism_event_label":"Inflammatory output fell in tissue studied outside the body.","subject":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"object":{"id":"7e4c9140-2c3e-5dae-ba2f-3302a9ab2d2f","slug":"il18","display_name":"Human interleukin 18 / IL18","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"4f264669-ed08-59a3-9a04-ca9d0101b831","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-placenta-il18-event","event_type":"observed_relationship","label":"Inflammatory output fell in tissue studied outside the body.","description":"Adding 1 mM ergothioneine for 24 hours reduced IL-18 production in placental explants from women with gestational diabetes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7e4c9140-2c3e-5dae-ba2f-3302a9ab2d2f","slug":"il18","display_name":"Human interleukin 18 / IL18","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"b53e832d-0776-5495-b5cd-cb6a8e8c538b","slug":"nlrp3","display_name":"NLRP3","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"a732d76c-af99-5440-b172-400274462345","slug":"nlrp3-inflammasome","display_name":"NLRP3 inflammasome","entity_type_key":"protein_complex"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human placental explants; comparison with NLRP3 inhibitor MCC950.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Ex vivo result, not a pregnancy intervention trial or proof of direct NLRP3 binding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Inflammatory output fell in tissue studied outside the body.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"L-ergothioneine reduces mitochondrial-driven NLRP3 activation in gestational diabetes mellitus. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38029485/ · DOI 10.1016/j.jri.2023.104171","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"60bf71cb-b6e7-5d0f-adc7-c8ff78d3df0d","evidence_kind":"source_excerpt","locator":"Lines 376-382","start_line":376,"end_line":382,"excerpt":"## ergothioneine-placenta-il18\nInflammatory output fell in tissue studied outside the body.\nAdding 1 mM ergothioneine for 24 hours reduced IL-18 production in placental explants from women with gestational diabetes.\nModel: Human placental explants; comparison with NLRP3 inhibitor MCC950.\nLimitations: Ex vivo result, not a pregnancy intervention trial or proof of direct NLRP3 binding.\nEvidence access: Primary abstract\nL-ergothioneine reduces mitochondrial-driven NLRP3 activation in gestational diabetes mellitus. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38029485/ · DOI 10.1016/j.jri.2023.104171","model_system":"Human placental explants; comparison with NLRP3 inhibitor MCC950.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b6def8119f03cfabeec2fb55ba924340dc612e1dd42f05404201fa2b7ced5259","revision_id":"f88c38e6-ffb5-5a68-83ca-4610ca2c2df4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}