{"id":"2401e6f4-0626-5c8d-a6e2-27ec90f02da0","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-inpp1-substrate-specific","predicate":"inhibits","statement":"Lithium inhibited bovine INPP1 differently by substrate: Ki about 6 mM for Ins(1,4)P2 versus 0.5–1 mM for Ins(1,3,4)P3.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"45955340-a530-51e3-86ba-e21bf3883e7b","mechanism_event_label":"The same enzyme can respond differently depending on its substrate.","subject":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"object":{"id":"49a701d3-dc96-5389-bafd-6948f38dc393","slug":"bovine-inpp1","display_name":"Bovine inositol polyphosphate 1-phosphatase / INPP1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"45955340-a530-51e3-86ba-e21bf3883e7b","stable_key":"b7798a90-72a3-5454-a321-c4be88bf0cc4:lithium-inpp1-substrate-specific-event","event_type":"observed_relationship","label":"The same enzyme can respond differently depending on its substrate.","description":"Lithium inhibited bovine INPP1 differently by substrate: Ki about 6 mM for Ins(1,4)P2 versus 0.5–1 mM for Ins(1,3,4)P3.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"13b4b09c-4581-58f6-9792-9a78125963e8","slug":"lithium-ion","display_name":"Lithium ion (Li+)","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"49a701d3-dc96-5389-bafd-6948f38dc393","slug":"bovine-inpp1","display_name":"Bovine inositol polyphosphate 1-phosphatase / INPP1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8e2e3902-5e26-52e3-9c85-1d7c0ace1637","slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"5b09f2d6-d3ce-50ee-80d7-a77680b379a2","slug":"inositol-1-4-bisphosphate","display_name":"Myo-inositol 1,4-bisphosphate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"7717d93b-5b8d-591a-a0ae-d7ee859f826d","slug":"inositol-1-3-4-trisphosphate","display_name":"Myo-inositol 1,3,4-trisphosphate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified calf-brain INPP1.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Do not substitute signaling IP3, Ins(1,4,5)P3, for the distinct Ins(1,3,4)P3 substrate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Lithium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"lithium","display_name":"Lithium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"The same enzyme can respond differently depending on its substrate.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inositol polyphosphate 1-phosphatase from calf brain. Purification and inhibition by Li+, Ca2+, and Mn2+. · 1987 · https://pubmed.ncbi.nlm.nih.gov/2824473/","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c06b975a-2f46-54b6-ad1b-51819a269cad","evidence_kind":"source_excerpt","locator":"Lines 56-62","start_line":56,"end_line":62,"excerpt":"## lithium-inpp1-substrate-specific\nThe same enzyme can respond differently depending on its substrate.\nLithium inhibited bovine INPP1 differently by substrate: Ki about 6 mM for Ins(1,4)P2 versus 0.5–1 mM for Ins(1,3,4)P3.\nModel: Purified calf-brain INPP1.\nLimitations: Do not substitute signaling IP3, Ins(1,4,5)P3, for the distinct Ins(1,3,4)P3 substrate.\nEvidence access: Primary abstract\nInositol polyphosphate 1-phosphatase from calf brain. Purification and inhibition by Li+, Ca2+, and Mn2+. · 1987 · https://pubmed.ncbi.nlm.nih.gov/2824473/","model_system":"Purified calf-brain INPP1.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b2dd2f3c-5c4c-5a12-9a0d-91b521ffcf98","stable_key":"import-b7798a90-72a3-5454-a321-c4be88bf0cc4","title":"Lithium: metal-sensitive enzymes, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"dc67e965ba78458cf2d88d000d687641d9104354115ab16529a3a64941bd777d","revision_id":"4af127bf-9ccc-516b-9481-fafd453c2c35","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}