{"id":"23741620-e114-5195-82fe-7e23534969b6","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-enzyme-neuronal-ne-accumulation","predicate":"increases","statement":"Adding 100 micromolar ascorbate to SH-SY5Y cultures increased intracellular norepinephrine over six hours in parallel with ascorbate accumulation.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"0dadfe96-785c-5e2c-83c1-676094a3465f","mechanism_event_label":"Vitamin C increased the neurotransmitter pool in these cultured neuronal cells.","subject":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"object":{"id":"34ab29ee-eb2b-5e51-9d25-0c1f8d5b97d9","slug":"norepinephrine","display_name":"Norepinephrine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"0dadfe96-785c-5e2c-83c1-676094a3465f","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-enzyme-neuronal-ne-accumulation-event","event_type":"observed_intervention","label":"Vitamin C increased the neurotransmitter pool in these cultured neuronal cells.","description":"Adding 100 micromolar ascorbate to SH-SY5Y cultures increased intracellular norepinephrine over six hours in parallel with ascorbate accumulation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"role":"supporting-reductant","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"34ab29ee-eb2b-5e51-9d25-0c1f8d5b97d9","slug":"norepinephrine","display_name":"Norepinephrine","entity_type_key":"small_molecule"},"role":"measured-product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Vitamin C chemistry in collagen, modified-lysine/carnitine metabolism or copper-dependent peptide/catecholamine processing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human SH-SY5Y neuroblastoma cultures","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"100 micromolar ascorbate added to human SH-SY5Y culture; time course through six hours and longer; Fig.1.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Figure 1 measures cellular content; it is not a purified DBH flux assay or demonstration of symptom improvement in people.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin C research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-c","display_name":"Vitamin C","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Vitamin C increased the neurotransmitter pool in these cultured neuronal cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[may2012] Mechanisms of ascorbic acid stimulation of norepinephrine synthesis in neuronal cells. (2012). https://pubmed.ncbi.nlm.nih.gov/22925890/ DOI: 10.1016/j.bbrc.2012.08.054","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Neuronal tumor cell line","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"eddc0ef0-8521-5a59-91a5-1ab4bdd803b8","evidence_kind":"source_excerpt","locator":"Lines 820-831","start_line":820,"end_line":831,"excerpt":"### vc-enzyme-neuronal-ne-accumulation\nAdding 100 micromolar ascorbate to SH-SY5Y cultures increased intracellular norepinephrine over six hours in parallel with ascorbate accumulation.\nCondition category: normal\nnutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Vitamin C increased the neurotransmitter pool in these cultured neuronal cells.\norganism: Homo sapiens\ntissue_or_cell_type: Neuronal tumor cell line\nexperimental_model: Human SH-SY5Y neuroblastoma cultures\nlimitations: Figure 1 measures cellular content; it is not a purified DBH flux assay or demonstration of symptom improvement in people.\ncross_nutrient: Vitamin C chemistry in collagen, modified-lysine/carnitine metabolism or copper-dependent peptide/catecholamine processing.\nexposure: 100 micromolar ascorbate added to human SH-SY5Y culture; time course through six hours and longer; Fig.1.\n[may2012] Mechanisms of ascorbic acid stimulation of norepinephrine synthesis in neuronal cells. 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