{"id":"23613337-e96b-5c40-88ec-12783abd19fb","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:lysine-deprivation-cellcycle","predicate":"withdrawal_inhibits","statement":"In noncancerous human cell lines, lysine withdrawal rapidly inhibited protein synthesis and stalled cell-cycle progression.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"952441f4-6ad9-5ae9-a681-311c5a1b4b29","mechanism_event_label":"Removing this required amino acid disrupted protein production and division in cultured cells.","subject":{"id":"5fff6664-209e-5c35-b88e-ac15c1c5fbc0","slug":"l-lysine","display_name":"L-Lysine","entity_type_key":"small_molecule"},"object":{"id":"4d7802c9-e18f-58d9-8bbd-ba41189ed614","slug":"protein-synthesis","display_name":"Protein synthesis","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"952441f4-6ad9-5ae9-a681-311c5a1b4b29","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:lysine-deprivation-cellcycle-event","event_type":"biochemical_relationship","label":"Removing this required amino acid disrupted protein production and division in cultured cells.","description":"In noncancerous human cell lines, lysine withdrawal rapidly inhibited protein synthesis and stalled cell-cycle progression.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5fff6664-209e-5c35-b88e-ac15c1c5fbc0","slug":"l-lysine","display_name":"L-Lysine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4d7802c9-e18f-58d9-8bbd-ba41189ed614","slug":"protein-synthesis","display_name":"Protein synthesis","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e588d159-6777-54e1-9a79-301dca680da2","slug":"cell-cycle-progression","display_name":"Cell-cycle progression","entity_type_key":"cellular_process"},"role":"affected_process","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"experimental_model","value_text":"Live-cell imaging and perturbation experiments, primarily MCF10A and RPE-hTERT","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Cell-line and acute-starvation context; response differs among amino acids and may vary across primary tissues.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Removing this required amino acid disrupted protein production and division in cultured cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[rong2023] Cells use multiple mechanisms for cell-cycle arrest upon withdrawal of individual amino acids (2023). https://pmc.ncbi.nlm.nih.gov/articles/PMC11238304/ DOI: 10.1016/j.celrep.2023.113539","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Mammary epithelial and retinal pigment epithelial cell lines","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"62eed6b7-6870-5030-bc4c-e842f8590141","evidence_kind":"source_excerpt","locator":"Lines 829-837","start_line":829,"end_line":837,"excerpt":"### lysine-deprivation-cellcycle\nIn noncancerous human cell lines, lysine withdrawal rapidly inhibited protein synthesis and stalled cell-cycle progression.\nPlain language: Removing this required amino acid disrupted protein production and division in cultured cells.\nCondition category: nutrient_deficiency\norganism: Homo sapiens\ntissue_or_cell_type: Mammary epithelial and retinal pigment epithelial cell lines\nexperimental_model: Live-cell imaging and perturbation experiments, primarily MCF10A and RPE-hTERT\nlimitations: Cell-line and acute-starvation context; response differs among amino acids and may vary across primary tissues.\n[rong2023] Cells use multiple mechanisms for cell-cycle arrest upon withdrawal of individual amino acids (2023). https://pmc.ncbi.nlm.nih.gov/articles/PMC11238304/ DOI: 10.1016/j.celrep.2023.113539","model_system":"Live-cell imaging and perturbation experiments, primarily MCF10A and RPE-hTERT","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [rong2023] Cells use multiple mechanisms for cell-cycle arrest upon withdrawal of individual amino acids (2023). https://pmc.ncbi.nlm.nih.gov/articles/PMC11238304/ DOI: 10.1016/j.celrep.2023.113539","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}