{"id":"232f68ac-3d21-5c7b-8471-9f0841db12fb","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-ra-homocysteine-response-boundary","predicate":"showed_nonsignificant_overall_challenge_trend","statement":"The overall post-methionine homocysteine treatment effect was a nonsignificant trend (P=0.086); an elevated-baseline subgroup showed a stronger signal.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"0682de55-a7d9-5c44-b29b-51c34e0c2532","mechanism_event_label":"The homocysteine test did not improve as consistently as the other B6 tests.","subject":{"id":"aa6648f2-8962-5567-9939-ef4145e40ed1","slug":"pyridoxine","display_name":"Pyridoxine","entity_type_key":"small_molecule"},"object":{"id":"5b635b42-bc0d-5c54-aa94-9cf76cd0ed47","slug":"homocysteine","display_name":"Homocysteine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"0682de55-a7d9-5c44-b29b-51c34e0c2532","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-ra-homocysteine-response-boundary-event","event_type":"observed_intervention","label":"The homocysteine test did not improve as consistently as the other B6 tests.","description":"The overall post-methionine homocysteine treatment effect was a nonsignificant trend (P=0.086); an elevated-baseline subgroup showed a stronger signal.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9d39f561-740b-5f67-bba7-8a72ef612a99","slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"},"role":"challenge_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"aa6648f2-8962-5567-9939-ef4145e40ed1","slug":"pyridoxine","display_name":"Pyridoxine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"5b635b42-bc0d-5c54-aa94-9cf76cd0ed47","slug":"homocysteine","display_name":"Homocysteine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Methionine handling depends on more than circulating B6.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Double-blind randomized placebo-controlled RA trial: 36 recruited, 33 baseline completers, 28 low-PLP patients eligible for supplementation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Pyridoxine hydrochloride 50 mg/day or placebo for 30 days; historical trial exposure, not a regimen recommendation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Subgroup findings are exploratory; methotrexate, inflammation and enzyme differences can affect the challenge.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The homocysteine test did not improve as consistently as the other B6 tests.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b6-chiang2005-trial] Pyridoxine supplementation corrects vitamin B6 deficiency but does not improve inflammation in patients with rheumatoid arthritis (2005). https://pubmed.ncbi.nlm.nih.gov/16277693/ DOI: 10.1186/ar1839","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Human blood and whole-body measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"63e98a05-d975-544d-bb2a-e6a317658c39","evidence_kind":"source_excerpt","locator":"Lines 1497-1508","start_line":1497,"end_line":1508,"excerpt":"### b6-ra-homocysteine-response-boundary\nThe overall post-methionine homocysteine treatment effect was a nonsignificant trend (P=0.086); an elevated-baseline subgroup showed a stronger signal.\nCondition category: biomarker_context\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The homocysteine test did not improve as consistently as the other B6 tests.\norganism: Homo sapiens\ntissue_or_cell_type: Human blood and whole-body measurements\nexperimental_model: Double-blind randomized placebo-controlled RA trial: 36 recruited, 33 baseline completers, 28 low-PLP patients eligible for supplementation.\nlimitations: Subgroup findings are exploratory; methotrexate, inflammation and enzyme differences can affect the challenge.\nexposure: Pyridoxine hydrochloride 50 mg/day or placebo for 30 days; historical trial exposure, not a regimen recommendation.\ncross_nutrient: Methionine handling depends on more than circulating B6.\n[b6-chiang2005-trial] Pyridoxine supplementation corrects vitamin B6 deficiency but does not improve inflammation in patients with rheumatoid arthritis (2005). https://pubmed.ncbi.nlm.nih.gov/16277693/ DOI: 10.1186/ar1839","model_system":"Double-blind randomized placebo-controlled RA trial: 36 recruited, 33 baseline completers, 28 low-PLP patients eligible for supplementation.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b6-chiang2005-trial] Pyridoxine supplementation corrects vitamin B6 deficiency but does not improve inflammation in patients with rheumatoid arthritis (2005). https://pubmed.ncbi.nlm.nih.gov/16277693/ DOI: 10.1186/ar1839","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}