{"id":"230a77b9-69da-544c-a24c-266165068364","stable_key":"c9aa15a1-8ba2-5913-b2dd-372af304bf87:fast-hmgcl","predicate":"cleaved_to","statement":"Human HMGCL cleaves HMG-CoA to acetoacetate and acetyl-CoA.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"73dd6fef-d274-5fcb-aa0a-a5250bb6a505","mechanism_event_label":"A second enzyme releases the first ketone body.","subject":{"id":"81e5bb47-0c11-54c9-a0ec-5c213930c173","slug":"hmg-coa","display_name":"(S)-3-Hydroxy-3-methylglutaryl-CoA","entity_type_key":"small_molecule"},"object":{"id":"1b77cb4a-1f79-50e2-b4f0-fd0953cb914e","slug":"acetoacetate","display_name":"Acetoacetate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"73dd6fef-d274-5fcb-aa0a-a5250bb6a505","stable_key":"c9aa15a1-8ba2-5913-b2dd-372af304bf87:fast-hmgcl-event","event_type":"observed_relationship","label":"A second enzyme releases the first ketone body.","description":"Human HMGCL cleaves HMG-CoA to acetoacetate and acetyl-CoA.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"81e5bb47-0c11-54c9-a0ec-5c213930c173","slug":"hmg-coa","display_name":"(S)-3-Hydroxy-3-methylglutaryl-CoA","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1b77cb4a-1f79-50e2-b4f0-fd0953cb914e","slug":"acetoacetate","display_name":"Acetoacetate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d2661983-120d-5a42-8ba3-454ab4727ed7","slug":"hmgcl","display_name":"Human HMG-CoA lyase / HMGCL","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b7ed6e4c-e560-5cee-b68f-895f21862e6f","slug":"acetyl-coa","display_name":"Acetyl-CoA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract and indexed full-text introduction, PMC2924059","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human HMGCL structural study; reaction in primary introduction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Mitochondrial pathway; net flux is not inferred from structure alone.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Fasting physiological-state collection; human protocols, cellular deprivation and refeeding are distinguished.","comparator":null,"unit":null,"notes":"","entity":{"slug":"fasting","display_name":"Fasting / abstention from energy intake","entity_type_key":"cellular_process"}},{"dimension":"plain_language","value_text":"A second enzyme releases the first ketone body.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Functional insights into human HMG-CoA lyase from structures of Acyl-CoA-containing ternary complexes. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20558737/ · DOI 10.1074/jbc.M110.139931","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b917c57b-4258-5b48-8438-834fb6387062","evidence_kind":"source_excerpt","locator":"Lines 112-118","start_line":112,"end_line":118,"excerpt":"## fast-hmgcl\nA second enzyme releases the first ketone body.\nHuman HMGCL cleaves HMG-CoA to acetoacetate and acetyl-CoA.\nModel: Human HMGCL structural study; reaction in primary introduction.\nLimitations: Mitochondrial pathway; net flux is not inferred from structure alone.\nEvidence access: Primary abstract and indexed full-text introduction, PMC2924059\nFunctional insights into human HMG-CoA lyase from structures of Acyl-CoA-containing ternary complexes. · 2010 · https://pubmed.ncbi.nlm.nih.gov/20558737/ · DOI 10.1074/jbc.M110.139931","model_system":"Human HMGCL structural study; reaction in primary introduction.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"921d1eb5-2c88-52b9-9898-60b2b5bf6029","stable_key":"import-c9aa15a1-8ba2-5913-b2dd-372af304bf87","title":"Fasting: fuel switching, nutrient sensing, ketone signaling, nutrient dependencies and refeeding (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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