{"id":"22e61a95-a942-593f-820a-0a023cb5f547","stable_key":"4d8e4781-3878-5818-8d0a-7dbd6e024555:chromium-cd36-trafficking","predicate":"promotes","statement":"Chromium picolinate increased plasma-membrane CD36 localization in 3T3-L1 adipocytes; combining it with insulin did not produce additive CD36 translocation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"ebf30363-050a-58c4-b257-31e9b8c4c60b","mechanism_event_label":"Chromium affected a fatty-acid transporter as well as GLUT4, with a different response pattern.","subject":{"id":"89d5d39c-2663-5e0c-8eae-562a5b25a47d","slug":"chromium-iii-picolinate","display_name":"Chromium(III) picolinate / CrPic","entity_type_key":"chemical_species"},"object":{"id":"9d8cc5fc-988b-5071-b062-c5fafcf75736","slug":"mouse-cd36-surface-translocation","display_name":"Mouse CD36 movement to the cell surface","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ebf30363-050a-58c4-b257-31e9b8c4c60b","stable_key":"4d8e4781-3878-5818-8d0a-7dbd6e024555:chromium-cd36-trafficking-event","event_type":"biochemical_relationship","label":"Chromium affected a fatty-acid transporter as well as GLUT4, with a different response pattern.","description":"Chromium picolinate increased plasma-membrane CD36 localization in 3T3-L1 adipocytes; combining it with insulin did not produce additive CD36 translocation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"98f1fe60-4f89-5d3f-830a-d1f61988a90c","slug":"mouse-cd36","display_name":"Mouse fatty-acid translocase / Cd36","entity_type_key":"protein"},"role":"transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d7ab69c0-cd7d-587c-b315-47af56493182","slug":"insulin","display_name":"Insulin","entity_type_key":"protein"},"role":"comparison_stimulus","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"89d5d39c-2663-5e0c-8eae-562a5b25a47d","slug":"chromium-iii-picolinate","display_name":"Chromium(III) picolinate / CrPic","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9d8cc5fc-988b-5071-b062-c5fafcf75736","slug":"mouse-cd36-surface-translocation","display_name":"Mouse CD36 movement to the cell surface","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chromium-research/20721637.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"550ce1e2898494fb6f385c11a446c6516812bffacefc4c78161fbee16adfc39f\", \"start_char\": 0, \"end_char\": 1671, \"text_sha256\": \"550ce1e2898494fb6f385c11a446c6516812bffacefc4c78161fbee16adfc39f\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Transporter localization and substrate-uptake assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Insulin, chromium picolinate and wortmannin comparisons","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Membrane localization is not equivalent to transporter activity. CD36 trafficking responses to insulin and chromium were not additive; results do not show a general increase in beneficial fatty-acid disposal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chromium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chromium","display_name":"Chromium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mouse 3T3-L1 adipocytes","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Chromium affected a fatty-acid transporter as well as GLUT4, with a different response pattern.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chromium-p20721637] Insulin and chromium picolinate induce translocation of CD36 to the plasma membrane through different signaling pathways in 3T3-L1 adipocytes, and with a differential functionality of the CD36. (2011). https://pubmed.ncbi.nlm.nih.gov/20721637/ DOI: 10.1007/s12011-010-8809-8","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Plasma membrane","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b2021073-fbf8-5e13-ac8e-3025311fd133","evidence_kind":"source_excerpt","locator":"Lines 458-469","start_line":458,"end_line":469,"excerpt":"### chromium-cd36-trafficking\nChromium picolinate increased plasma-membrane CD36 localization in 3T3-L1 adipocytes; combining it with insulin did not produce additive CD36 translocation.\nCondition category: normal\nnutrient_topic: Chromium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Chromium affected a fatty-acid transporter as well as GLUT4, with a different response pattern.\norganism: Mouse 3T3-L1 adipocytes\ntissue_or_cell_type: Plasma membrane\nexperimental_model: Transporter localization and substrate-uptake assays\nlimitations: Membrane localization is not equivalent to transporter activity. CD36 trafficking responses to insulin and chromium were not additive; results do not show a general increase in beneficial fatty-acid disposal.\nexposure: Insulin, chromium picolinate and wortmannin comparisons\nevidence_span: {\"source_cache\": \"artifacts/chromium-research/20721637.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"550ce1e2898494fb6f385c11a446c6516812bffacefc4c78161fbee16adfc39f\", \"start_char\": 0, \"end_char\": 1671, \"text_sha256\": \"550ce1e2898494fb6f385c11a446c6516812bffacefc4c78161fbee16adfc39f\"}\n[chromium-p20721637] Insulin and chromium picolinate induce translocation of CD36 to the plasma membrane through different signaling pathways in 3T3-L1 adipocytes, and with a differential functionality of the CD36. (2011). https://pubmed.ncbi.nlm.nih.gov/20721637/ DOI: 10.1007/s12011-010-8809-8","model_system":"Transporter localization and substrate-uptake assays","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [chromium-p20721637] Insulin and chromium picolinate induce translocation of CD36 to the plasma membrane through different signaling pathways in 3T3-L1 adipocytes, and with a differential functionality of the CD36. 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