{"id":"22e56f52-80fd-507b-bb08-622a74ce83c1","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-lrat-chylomicron-retinoids","predicate":"determines-composition-of","statement":"Lrat-null mice still secreted chylomicron retinyl esters whose acyl composition reflected the gavage oil.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"8e6aa247-bc2e-545b-b4d6-c7bd8397df2b","mechanism_event_label":"Alternative esterification partly handles absorbed retinol when LRAT is missing.","subject":{"id":"f7ce5851-df6d-54ed-b467-482480d26ff9","slug":"lrat","display_name":"Lecithin:retinol acyltransferase / LRAT","entity_type_key":"protein"},"object":{"id":"5cf2b51c-2075-50ff-b8e8-1b32f369e6a3","slug":"chylomicron-retinoid-delivery","display_name":"Chylomicron retinoid delivery","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8e6aa247-bc2e-545b-b4d6-c7bd8397df2b","stable_key":"dff9f743-3766-5f20-a71e-d6d2bd5bfb6f:va-lrat-chylomicron-retinoids-event","event_type":"biochemical_relationship","label":"Alternative esterification partly handles absorbed retinol when LRAT is missing.","description":"Lrat-null mice still secreted chylomicron retinyl esters whose acyl composition reflected the gavage oil.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"148c57d8-c658-5769-89e8-1194cef2a782","slug":"retinol","display_name":"All-trans-retinol","entity_type_key":"small_molecule"},"role":"oral-substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"62e3f5bd-503b-5ea8-b102-966aa3b7319c","slug":"retinyl-palmitate","display_name":"All-trans-retinyl palmitate","entity_type_key":"small_molecule"},"role":"measured-ester","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"83cbd152-263c-51ab-a716-cc762c0948e4","slug":"retinyl-oleate","display_name":"All-trans-retinyl oleate","entity_type_key":"small_molecule"},"role":"measured-ester","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"7d1ed080-f93e-5248-9b45-7aa30228fd35","slug":"retinyl-linoleate","display_name":"All-trans-retinyl linoleate","entity_type_key":"small_molecule"},"role":"measured-ester","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"52a8dc1b-c938-547b-bf75-b2f378b2272e","slug":"lrat-loss","display_name":"LRAT loss","entity_type_key":"protein_state"},"role":"experimental-machinery-state","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"f7ce5851-df6d-54ed-b467-482480d26ff9","slug":"lrat","display_name":"Lecithin:retinol acyltransferase / LRAT","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"5cf2b51c-2075-50ff-b8e8-1b32f369e6a3","slug":"chylomicron-retinoid-delivery","display_name":"Chylomicron retinoid delivery","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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unverified.","entity":null}],"evidence":[{"id":"a20e756a-0afe-5b78-850d-fb71760f842e","evidence_kind":"source_excerpt","locator":"Lines 346-358","start_line":346,"end_line":358,"excerpt":"### va-lrat-chylomicron-retinoids\nLrat-null mice still secreted chylomicron retinyl esters whose acyl composition reflected the gavage oil.\nCondition category: machinery_impairment\nnutrient_topic: Vitamin A research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Alternative esterification partly handles absorbed retinol when LRAT is missing.\norganism: Mus musculus\ntissue_or_cell_type: Intestine and isolated chylomicrons\nexperimental_model: Lrat-knockout mice; retinol gavage and one-month retinoid-deficient diet.\nlimitations: Clearance was pharmacologically blocked; remaining enzyme identity was not established here.\nexposure: 6 micrograms retinol in peanut oil; P-407 clearance blockade; sampling at three hours.\noutcome: Lrat-null mice still secreted chylomicron retinyl esters whose acyl composition reflected the gavage oil.\nevidence_location: Figure 3\n[va-obyrne-2005] Retinoid Absorption and Storage Is Impaired in Mice Lacking Lecithin:Retinol Acyltransferase (LRAT) (2005). https://pmc.ncbi.nlm.nih.gov/articles/PMC1352312/ DOI: 10.1074/jbc.M507924200","model_system":"Lrat-knockout mice; 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