{"id":"22e2f131-1185-5a91-ae9a-6fd71d9ad551","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-mrp-retention","predicate":"reduced","statement":"MRP1 expression reduced intracellular sulforaphane/conjugate accumulation and attenuated ARE-dependent responses.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5d9da4dd-b78e-5cb1-af0f-f76f9d3eee10","mechanism_event_label":"Export can counter the retention effect.","subject":{"id":"537adebd-e04d-5859-b5e3-5b054359c20f","slug":"abcc1","display_name":"Human multidrug resistance-associated protein 1 / ABCC1 / MRP1","entity_type_key":"protein"},"object":{"id":"5b74cc38-d5d1-50bd-8542-8d9b3c570df5","slug":"sulforaphane-intracellular-accumulation","display_name":"Intracellular sulforaphane plus glutathione-conjugate accumulation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5d9da4dd-b78e-5cb1-af0f-f76f9d3eee10","stable_key":"44eaeae5-557a-552b-8998-884f30462e2a:sulforaphane-mrp-retention-event","event_type":"biochemical_relationship","label":"Export can counter the retention effect.","description":"MRP1 expression reduced intracellular sulforaphane/conjugate accumulation and attenuated ARE-dependent responses.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"role":"exposure","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"conjugating_substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e91e547a-a84c-5d7d-afbd-89e38a950c16","slug":"sulforaphane-glutathione","display_name":"Sulforaphane-glutathione conjugate / SFN-GSH","entity_type_key":"small_molecule"},"role":"conjugate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"537adebd-e04d-5859-b5e3-5b054359c20f","slug":"abcc1","display_name":"Human multidrug resistance-associated protein 1 / ABCC1 / MRP1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"5b74cc38-d5d1-50bd-8542-8d9b3c570df5","slug":"sulforaphane-intracellular-accumulation","display_name":"Intracellular sulforaphane plus glutathione-conjugate accumulation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/sulforaphane-research/18204073.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"0fcd98974f9fe12b2ca7c4326f94eddf4f20de4ac2ae6fdebb64de0efb1d8be9\", \"start_char\": 0, \"end_char\": 1894, \"text_sha256\": \"0fcd98974f9fe12b2ca7c4326f94eddf4f20de4ac2ae6fdebb64de0efb1d8be9\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Transgenic transporter/enzyme comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Sulforaphane; GSH depletion control","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Engineered cancer cells; enzyme expression and efflux can have opposing effects without being a scientific contradiction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sulforaphane research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Human MCF7 cells with GSTP1 and/or MRP1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Export can counter the retention effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sulforaphane-p18204073] Expression of MRP1 and GSTP1-1 modulate the acute cellular response to treatment with the chemopreventive isothiocyanate, sulforaphane. 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