{"id":"22809bd2-c30d-502b-bed8-99ad376a0326","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:p6c-acetoacetate-2opp","predicate":"reacts_to_form","statement":"P6C and acetoacetate form 2-OPP in chemical incubations including plasma.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"bf1beebc-c47e-56eb-8945-3e25ae8c0190","mechanism_event_label":"An accumulated intermediate can react with a ketone body.","subject":{"id":"9a7b1d83-ebb2-5dea-b4a7-c040502fba4d","slug":"piperideine-6-carboxylate","display_name":"Delta1-piperideine-6-carboxylate","entity_type_key":"small_molecule"},"object":{"id":"80400fee-4e65-5cf7-a5a6-1cd3f93ef1ac","slug":"2-opp","display_name":"2S,6S-/2S,6R-6-(2-Oxopropyl)piperidine-2-carboxylate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"bf1beebc-c47e-56eb-8945-3e25ae8c0190","stable_key":"c3df3634-4c3a-5099-a5d9-e4f6344c1084:p6c-acetoacetate-2opp-event","event_type":"biochemical_relationship","label":"An accumulated intermediate can react with a ketone body.","description":"P6C and acetoacetate form 2-OPP in chemical incubations including plasma.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"9a7b1d83-ebb2-5dea-b4a7-c040502fba4d","slug":"piperideine-6-carboxylate","display_name":"Delta1-piperideine-6-carboxylate","entity_type_key":"small_molecule"},"role":"reactant","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1b77cb4a-1f79-50e2-b4f0-fd0953cb914e","slug":"acetoacetate","display_name":"Acetoacetate","entity_type_key":"small_molecule"},"role":"reactant","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"80400fee-4e65-5cf7-a5a6-1cd3f93ef1ac","slug":"2-opp","display_name":"2S,6S-/2S,6R-6-(2-Oxopropyl)piperidine-2-carboxylate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"affected_machinery","value_text":"ALDH7A1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"deficiency_not_equivalent","value_text":"Dietary lysine deficiency","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Chemical incubations in aqueous solutions and human biological matrices","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"In-vivo formation is inferred; patient flux was not measured.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"An accumulated intermediate can react with a ketone body.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[engelke2021] Untargeted metabolomics and infrared ion spectroscopy identify biomarkers for pyridoxine-dependent epilepsy (2021). https://www.jci.org/articles/view/148272 DOI: 10.1172/JCI148272","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Body-fluid reaction model","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"d6a52670-1471-5b65-9b2a-6bb650099f0d","evidence_kind":"source_excerpt","locator":"Lines 302-312","start_line":302,"end_line":312,"excerpt":"### p6c-acetoacetate-2opp\nP6C and acetoacetate form 2-OPP in chemical incubations including plasma.\nPlain language: An accumulated intermediate can react with a ketone body.\nCondition category: machinery_impairment\norganism: Homo sapiens\ntissue_or_cell_type: Body-fluid reaction model\nexperimental_model: Chemical incubations in aqueous solutions and human biological matrices\nlimitations: In-vivo formation is inferred; patient flux was not measured.\naffected_machinery: ALDH7A1\ndeficiency_not_equivalent: Dietary lysine deficiency\n[engelke2021] Untargeted metabolomics and infrared ion spectroscopy identify biomarkers for pyridoxine-dependent epilepsy (2021). https://www.jci.org/articles/view/148272 DOI: 10.1172/JCI148272","model_system":"Chemical incubations in aqueous solutions and human biological matrices","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact quote from the accompanying curation document, not from publisher text. Original study references: [engelke2021] Untargeted metabolomics and infrared ion spectroscopy identify biomarkers for pyridoxine-dependent epilepsy (2021). https://www.jci.org/articles/view/148272 DOI: 10.1172/JCI148272","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7633bde7-dcc9-5086-91c6-a45eb96857f3","stable_key":"import-c3df3634-4c3a-5099-a5d9-e4f6344c1084","title":"L-Lysine: mechanism-first literature curation (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"93998d47c21525409ba82f1c82ededf2893dff15fbe61c7deffc175b0e298e97","revision_id":"3897e31f-6624-59e1-a053-8a81b7361632","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}