{"id":"2211f7d4-ff73-5aa4-8304-4685c440e982","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:gart-formyl-transfer","predicate":"formylates","statement":"The human GART transformylase domain binds a folate formyl donor and GAR-site acceptor in a ternary complex supporting GAR formylation to FGAR.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"372bcfda-a78d-5cdb-9390-388198b04729","mechanism_event_label":"Folate donates one carbon early in purine-base construction.","subject":{"id":"95f9203c-16c4-5c62-bd21-0b268e228a08","slug":"gart","display_name":"Human trifunctional GART","entity_type_key":"protein"},"object":{"id":"dcc0d839-c860-53c4-8d06-6592ad195af4","slug":"gar","display_name":"Glycinamide ribonucleotide","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"372bcfda-a78d-5cdb-9390-388198b04729","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:gart-formyl-transfer-event","event_type":"biochemical_relationship","label":"Folate donates one carbon early in purine-base construction.","description":"The human GART transformylase domain binds a folate formyl donor and GAR-site acceptor in a ternary complex supporting GAR formylation to FGAR.","status":"provisional","compartment":{"slug":"cytosol","display_name":"Cytosol"},"participants":[{"entity":{"id":"ec73b364-a2ea-5c4a-bcea-a54aeebf57d1","slug":"10-formyltetrahydrofolate","display_name":"10-Formyltetrahydrofolate","entity_type_key":"small_molecule"},"role":"physiological donor represented by analogue","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"55740a62-b086-5e66-8b47-7764ff68fd8c","slug":"fgar","display_name":"Formylglycinamide ribonucleotide","entity_type_key":"small_molecule"},"role":"physiological product","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"d2b23343-c688-5fc3-9f42-bfac0050552b","slug":"tetrahydrofolate","display_name":"Tetrahydrofolate","entity_type_key":"small_molecule"},"role":"physiological folate coproduct","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"95f9203c-16c4-5c62-bd21-0b268e228a08","slug":"gart","display_name":"Human trifunctional GART","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"dcc0d839-c860-53c4-8d06-6592ad195af4","slug":"gar","display_name":"Glycinamide ribonucleotide","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"X-ray crystallography","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Assay conditions described in the linked primary study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Structures used 10-formyl-dideazafolate and hydroxyacetamide ribonucleotide analogues.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"folate","display_name":"Folate (vitamin B9)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Folate donates one carbon early in purine-base construction.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[dahms-2005] The apo and ternary complex structures of a chemotherapeutic target: human glycinamide ribonucleotide transformylase (2005). https://pubmed.ncbi.nlm.nih.gov/16026156/ DOI: 10.1021/bi050307g","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified domain","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"b4286167-2fa0-531d-8c74-9d869482be1f","evidence_kind":"source_excerpt","locator":"Lines 998-1008","start_line":998,"end_line":1008,"excerpt":"### gart-formyl-transfer\nThe human GART transformylase domain binds a folate formyl donor and GAR-site acceptor in a ternary complex supporting GAR formylation to FGAR.\nCondition category: normal\nnutrient_topic: Folate and folic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Folate donates one carbon early in purine-base construction.\norganism: Homo sapiens\ntissue_or_cell_type: Purified domain\nexperimental_model: X-ray crystallography\nlimitations: Structures used 10-formyl-dideazafolate and hydroxyacetamide ribonucleotide analogues.\nexposure: Assay conditions described in the linked primary study.\n[dahms-2005] The apo and ternary complex structures of a chemotherapeutic target: human glycinamide ribonucleotide transformylase (2005). https://pubmed.ncbi.nlm.nih.gov/16026156/ DOI: 10.1021/bi050307g","model_system":"X-ray crystallography","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [dahms-2005] The apo and ternary complex structures of a chemotherapeutic target: human glycinamide ribonucleotide transformylase (2005). https://pubmed.ncbi.nlm.nih.gov/16026156/ DOI: 10.1021/bi050307g","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}