{"id":"2200b1d9-b7df-52d4-9bb0-87a5148bb37b","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-gout-nlrp3","predicate":"decreases_in_recorded_comparison","statement":"Apigenin reduced NLRP3 expression and accompanying caspase-1 activation and IL-1β release in the urate-crystal macrophage model.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"6cdb0579-2363-5993-b2cf-cdc3a0b94dbc","mechanism_event_label":"Apigenin reduced NLRP3 expression and accompanying caspase-1 activation and IL-1β release in the urate-crystal macrophage model.","subject":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"object":{"id":"ae25f14e-2a54-5094-9952-a97b718f94a3","slug":"mouse-nlrp3","display_name":"Mouse NLRP3","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"6cdb0579-2363-5993-b2cf-cdc3a0b94dbc","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-gout-nlrp3-event","event_type":"experimental_observation","label":"Apigenin reduced NLRP3 expression and accompanying caspase-1 activation and IL-1β release in the urate-crystal macrophage model.","description":"### api-gout-nlrp3\n\nApigenin reduced NLRP3 expression and accompanying caspase-1 activation and IL-1β release in the urate-crystal macrophage model.\nCD38 knockout/inhibitor comparisons in this study support a contribution in this model, not unique mediation across all stimuli.\nModel and exposure: MSU-stimulated mouse BMDMs; longer exposure than the 2025 post-priming experiments.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: The NADase CD38 is a central regulator in gouty inflammation and a novel druggable therapeutic target. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38493256/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11058052/ | https://doi.org/10.1007/s00011-024-01863-y | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Apigenin plus MSU","sequence_order":0,"notes":""},{"entity":{"id":"ae25f14e-2a54-5094-9952-a97b718f94a3","slug":"mouse-nlrp3","display_name":"Mouse NLRP3","entity_type_key":"protein"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"c17455e2-5805-55e7-9ecc-09c953115c84","slug":"mouse-il1b","display_name":"Mouse IL-1 beta","entity_type_key":"protein"},"role":"released cytokine","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"Apigenin plus MSU","comparator":"MSU alone","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"0.2 mg/mL MSU challenge","comparator":"MSU alone","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"monosodium-urate-crystals","display_name":"Monosodium urate crystals","entity_type_key":"small_molecule"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Apigenin plus MSU\", \"comparator\": \"MSU alone\", \"endpoint\": \"Apigenin reduced NLRP3 expression and accompanying caspase-1 activation and IL-1β release in the urate-crystal macrophage model.\", \"effect_direction\": \"decrease\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"apigenin\", \"state\": \"Apigenin plus MSU\"}, {\"entity_slug\": \"monosodium-urate-crystals\", \"state\": \"0.2 mg/mL MSU challenge\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"MSU-stimulated mouse BMDMs; longer exposure than the 2025 post-priming experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Apigenin reduced NLRP3 expression and accompanying caspase-1 activation and IL-1β release in the urate-crystal macrophage model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The NADase CD38 is a central regulator in gouty inflammation and a novel druggable therapeutic target. | https://pubmed.ncbi.nlm.nih.gov/38493256/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11058052/ | https://doi.org/10.1007/s00011-024-01863-y","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-gout-nlrp3; CD38 knockout/inhibitor comparisons in this study support a contribution in this model, not unique mediation across all stimuli.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8fce37d0-b35b-5de2-a963-8ce0efc9f1ad","evidence_kind":"source_excerpt","locator":"Lines 324-330","start_line":324,"end_line":330,"excerpt":"### api-gout-nlrp3\n\nApigenin reduced NLRP3 expression and accompanying caspase-1 activation and IL-1β release in the urate-crystal macrophage model.\nCD38 knockout/inhibitor comparisons in this study support a contribution in this model, not unique mediation across all stimuli.\nModel and exposure: MSU-stimulated mouse BMDMs; longer exposure than the 2025 post-priming experiments.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: The NADase CD38 is a central regulator in gouty inflammation and a novel druggable therapeutic target. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38493256/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11058052/ | https://doi.org/10.1007/s00011-024-01863-y | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"MSU-stimulated mouse BMDMs; longer exposure than the 2025 post-priming experiments.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[{"id":"6cadb362-c667-5b9a-ac17-a5ed8d5d3c9f","relation_type":"limits_scope_of","notes":"The 2025 genetic experiment limits universal CD38 mediation. Different priming, duration and stimuli need direct matched comparison.","source_claim_id":"cb9a714d-03ec-5f34-acff-4067f4c36150","source_claim_key":"ff370329-68a3-573d-96ee-597de78a3967:api-mouse-cd38-independent","source_statement":"Apigenin still suppressed nigericin-induced IL-1β release in CD38-knockout mouse macrophages, with a similar pattern to wild-type cells.","target_claim_id":"2200b1d9-b7df-52d4-9bb0-87a5148bb37b","target_claim_key":"ff370329-68a3-573d-96ee-597de78a3967:api-gout-nlrp3","target_statement":"Apigenin reduced NLRP3 expression and accompanying caspase-1 activation and IL-1β release in the urate-crystal macrophage model."}],"conflicts":[],"corrections":[],"research":null}