{"id":"21e8d4f7-eca9-5c11-b87d-af733bf7c78c","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ver-diet-e-muscle-repair-failure","predicate":"supports","statement":"Following 11 months on vitamin E-stripped chow, rat flexor digitorum brevis fibers displayed continued dye influx after controlled laser injury; normal chow and alpha-tocopherol-add-back controls resealed more effectively.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"50e62d88-c382-5423-a43f-50b4cd9d7a0d","mechanism_event_label":"Long-term vitamin E deprivation impaired muscle-fiber wound repair in rats.","subject":{"id":"40a6525a-76b8-576e-b73d-1e11aecf5f8d","slug":"alpha-tocopherol","display_name":"Alpha-tocopherol","entity_type_key":"small_molecule"},"object":{"id":"19860ac9-fc80-51bb-9bee-33f193616b4c","slug":"plasma-membrane-repair","display_name":"Plasma membrane repair","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"50e62d88-c382-5423-a43f-50b4cd9d7a0d","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ver-diet-e-muscle-repair-failure-event","event_type":"biochemical_relationship","label":"Long-term vitamin E deprivation impaired muscle-fiber wound repair in rats.","description":"Following 11 months on vitamin E-stripped chow, rat flexor digitorum brevis fibers displayed continued dye influx after controlled laser injury; normal chow and alpha-tocopherol-add-back controls resealed more effectively.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f0b6bcb6-5d41-5d9d-af9f-6af1a94d5c5a","slug":"plasma-membrane","display_name":"Plasma membrane","entity_type_key":"organelle"},"role":"injured myofiber membrane","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"40a6525a-76b8-576e-b73d-1e11aecf5f8d","slug":"alpha-tocopherol","display_name":"Alpha-tocopherol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"19860ac9-fc80-51bb-9bee-33f193616b4c","slug":"plasma-membrane-repair","display_name":"Plasma membrane repair","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Methods; Figure 3","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Dietary depletion with chow and add-back controls; laser assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Male Sprague-Dawley rats started diets at 4 weeks; assay at 11-month diet interval.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Long-term rat dietary deprivation and controlled wound assays; not evidence that ordinary supplementation improves muscle performance in replete people.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin E research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-e","display_name":"Vitamin E","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Long-term vitamin E deprivation impaired muscle-fiber wound repair in rats.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ver-labazi2015] The antioxidant requirement for plasma membrane repair in skeletal muscle. 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