{"id":"21c51464-c867-5764-9b8b-5460400e84bb","stable_key":"8c946991-97f0-55ee-8df0-00e67621962f:nad-plus-microbial-deamidation","predicate":"converts_nicotinamide_to","statement":"Bacterial PncA converted nicotinamide to nicotinic acid, enabling an alternative deamidated NAD synthesis route in mammalian cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f5132fb5-4264-509c-9bb5-b109f553419b","mechanism_event_label":"Microbes can redirect one vitamin form into another route around a blocked enzyme.","subject":{"id":"7944df5f-b616-55c0-8563-edf3943dbf7d","slug":"escherichia-coli-pnca","display_name":"Escherichia coli nicotinamidase PncA","entity_type_key":"protein"},"object":{"id":"9751fdb6-9b30-586f-b07a-79b8e2cd62dd","slug":"nicotinic-acid","display_name":"Nicotinic acid","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"f5132fb5-4264-509c-9bb5-b109f553419b","stable_key":"8c946991-97f0-55ee-8df0-00e67621962f:nad-plus-microbial-deamidation-event","event_type":"observed_relationship","label":"Microbes can redirect one vitamin form into another route around a blocked enzyme.","description":"Bacterial PncA converted nicotinamide to nicotinic acid, enabling an alternative deamidated NAD synthesis route in mammalian cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7944df5f-b616-55c0-8563-edf3943dbf7d","slug":"escherichia-coli-pnca","display_name":"Escherichia coli nicotinamidase PncA","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"9751fdb6-9b30-586f-b07a-79b8e2cd62dd","slug":"nicotinic-acid","display_name":"Nicotinic acid","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"08c27a3f-552e-5c92-8688-321a9b64a0d6","slug":"nicotinamide","display_name":"Nicotinamide","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"ee8024b9-b9e9-513f-9bcd-b3d30e1990ba","slug":"naprt","display_name":"Human nicotinate phosphoribosyltransferase / NAPRT","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"E. coli PncA genetic experiments, mammalian cancer cells and xenografts.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"PncA is bacterial; do not create a human nicotinamidase from this result.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"NAD+ collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Microbes can redirect one vitamin form into another route around a blocked enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Bacteria Boost Mammalian Host NAD Metabolism by Engaging the Deamidated Biosynthesis Pathway. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32130883/ · DOI 10.1016/j.cmet.2020.02.001","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"3662164f-ea33-5738-951e-349cccb6e4f2","evidence_kind":"source_excerpt","locator":"Lines 164-170","start_line":164,"end_line":170,"excerpt":"## nad-plus-microbial-deamidation\nMicrobes can redirect one vitamin form into another route around a blocked enzyme.\nBacterial PncA converted nicotinamide to nicotinic acid, enabling an alternative deamidated NAD synthesis route in mammalian cells.\nModel: E. coli PncA genetic experiments, mammalian cancer cells and xenografts.\nLimitations: PncA is bacterial; do not create a human nicotinamidase from this result.\nEvidence access: Primary full text\nBacteria Boost Mammalian Host NAD Metabolism by Engaging the Deamidated Biosynthesis Pathway. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32130883/ · DOI 10.1016/j.cmet.2020.02.001","model_system":"E. coli PncA genetic experiments, mammalian cancer cells and xenografts.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f59aa302-d463-5462-876f-e21e64861eac","stable_key":"import-8c946991-97f0-55ee-8df0-00e67621962f","title":"NAD+: compartmental supply, consumption and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"9f5324d0d652c80ad630b3100e28ce34cd2ce260ea2e1324332a4262ce72ae09","revision_id":"0e07909c-e8bc-5950-bafc-61371d9d6412","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}