{"id":"2188216e-2371-549c-a850-41999d1f32f6","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-microglia-nfkb-null","predicate":"does_not_reduce","statement":"Luteolin did not reduce LPS-induced NF-kappaB DNA binding or I-kappaB-alpha degradation in this microglial experiment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"46e2a199-a66d-5b8b-a99e-a9a7a7872153","mechanism_event_label":"Anti-inflammatory activity need not mean every pathway is blocked.","subject":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"object":{"id":"656c748f-e08a-5b69-83bd-221aa5ac485b","slug":"mouse-microglial-nfkb-activity","display_name":"Mouse microglial NF-kappaB DNA-binding activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"46e2a199-a66d-5b8b-a99e-a9a7a7872153","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-microglia-nfkb-null-event","event_type":"observed_relationship","label":"Anti-inflammatory activity need not mean every pathway is blocked.","description":"Luteolin did not reduce LPS-induced NF-kappaB DNA binding or I-kappaB-alpha degradation in this microglial experiment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"656c748f-e08a-5b69-83bd-221aa5ac485b","slug":"mouse-microglial-nfkb-activity","display_name":"Mouse microglial NF-kappaB DNA-binding activity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse BV-2 cells with LPS.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Different stimuli or timings can yield different responses.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Luteolin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Anti-inflammatory activity need not mean every pathway is blocked.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Luteolin reduces IL-6 production in microglia by inhibiting JNK phosphorylation and activation of AP-1. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18490655/ · DOI 10.1073/pnas.0802865105","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0918f658-bfd6-5188-801f-4e6daf4684f2","evidence_kind":"source_excerpt","locator":"Lines 412-418","start_line":412,"end_line":418,"excerpt":"## luteolin-microglia-nfkb-null\nAnti-inflammatory activity need not mean every pathway is blocked.\nLuteolin did not reduce LPS-induced NF-kappaB DNA binding or I-kappaB-alpha degradation in this microglial experiment.\nModel: Mouse BV-2 cells with LPS.\nLimitations: Different stimuli or timings can yield different responses.\nEvidence access: Primary abstract\nLuteolin reduces IL-6 production in microglia by inhibiting JNK phosphorylation and activation of AP-1. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18490655/ · DOI 10.1073/pnas.0802865105","model_system":"Mouse BV-2 cells with LPS.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"26337a08-5cee-5cee-900e-e6d3e26ac6e4","stable_key":"import-22527305-af1f-536a-b419-7e73f08cc3ec","title":"Luteolin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"fd6ea0b1cef8d1c9965270494bfa815de14f679044f0b5d5d7bbf4904630ad76","revision_id":"c9a3dd80-c7e6-5fb4-bc91-8db265e7c8de","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}