{"id":"2174432f-a9ac-5305-a1e9-ec57fd49d960","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-deficiency-insr-autophosphorylation","predicate":"low_supply_impairs","statement":"Insulin receptor autophosphorylation was reduced about 50% in muscle preparations from Mg-deficient rats, despite similar insulin binding.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"d9b3082b-622f-5be7-813a-340a2fd09ae4","mechanism_event_label":"The receptor could still bind insulin, but its downstream activation was impaired.","subject":{"id":"1f0c42f0-eaf3-5b86-966d-7ffc8a84872b","slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"},"object":{"id":"8b957cf2-0b40-5f1f-acd7-a0cc0349d2b0","slug":"insulin-receptor-autophosphorylation","display_name":"Insulin receptor autophosphorylation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d9b3082b-622f-5be7-813a-340a2fd09ae4","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:mg-deficiency-insr-autophosphorylation-event","event_type":"biochemical_relationship","label":"The receptor could still bind insulin, but its downstream activation was impaired.","description":"Insulin receptor autophosphorylation was reduced about 50% in muscle preparations from Mg-deficient rats, despite similar insulin binding.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5cd18880-8dc7-5897-9362-7422684f5f79","slug":"insr","display_name":"Insulin receptor / INSR","entity_type_key":"protein"},"role":"measured_enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d7ab69c0-cd7d-587c-b315-47af56493182","slug":"insulin","display_name":"Insulin","entity_type_key":"protein"},"role":"ligand","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"1f0c42f0-eaf3-5b86-966d-7ffc8a84872b","slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8b957cf2-0b40-5f1f-acd7-a0cc0349d2b0","slug":"insulin-receptor-autophosphorylation","display_name":"Insulin receptor autophosphorylation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"cross_nutrient","value_text":"Magnesium -> insulin signaling -> carbohydrate handling.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Partially purified rat gastrocnemius receptors.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A depletion experiment; not isolated Mg binding to a particular receptor site.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The receptor could still bind insulin, but its downstream activation was impaired.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mg-suarez1995] Impaired tyrosine-kinase activity of muscle insulin receptors from hypomagnesaemic rats (1995). https://pubmed.ncbi.nlm.nih.gov/8582534/ DOI: 10.1007/bf00401757","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Rat gastrocnemius receptor preparations and perfused hindquarter","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"nutrient_deficiency","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"1a170039-3dcd-5009-a497-80b62bcfa663","evidence_kind":"source_excerpt","locator":"Lines 1503-1513","start_line":1503,"end_line":1513,"excerpt":"### mg-deficiency-insr-autophosphorylation\nInsulin receptor autophosphorylation was reduced about 50% in muscle preparations from Mg-deficient rats, despite similar insulin binding.\nCondition category: nutrient_deficiency\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The receptor could still bind insulin, but its downstream activation was impaired.\norganism: Rattus norvegicus\ntissue_or_cell_type: Rat gastrocnemius receptor preparations and perfused hindquarter\nexperimental_model: Partially purified rat gastrocnemius receptors.\nlimitations: A depletion experiment; not isolated Mg binding to a particular receptor site.\ncross_nutrient: Magnesium -> insulin signaling -> carbohydrate handling.\n[mg-suarez1995] Impaired tyrosine-kinase activity of muscle insulin receptors from hypomagnesaemic rats (1995). https://pubmed.ncbi.nlm.nih.gov/8582534/ DOI: 10.1007/bf00401757","model_system":"Partially purified rat gastrocnemius receptors.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mg-suarez1995] Impaired tyrosine-kinase activity of muscle insulin receptors from hypomagnesaemic rats (1995). https://pubmed.ncbi.nlm.nih.gov/8582534/ DOI: 10.1007/bf00401757","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}