{"id":"2163edfd-e63f-5d20-8152-27e16e6331c2","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-immune-stimulated","predicate":"suppresses","statement":"Stevioside at 1 mM suppressed LPS-induced TNF and IL-1beta release in human THP-1 cells, with suppression of IKKbeta and NF-kappaB activation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"da1c2922-14cc-5d3f-8621-dc04fab206d2","mechanism_event_label":"The inflammatory state changes what the compound does.","subject":{"id":"06e03b41-c225-5fa3-8aa3-13a07a9826a9","slug":"stevioside","display_name":"Stevioside","entity_type_key":"small_molecule"},"object":{"id":"3d8d3010-7667-5664-b73d-e9f7199d6c10","slug":"human-thp1-lps-cytokine-release","display_name":"Human THP-1 LPS-induced cytokine release","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"da1c2922-14cc-5d3f-8621-dc04fab206d2","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-immune-stimulated-event","event_type":"observed_relationship","label":"The inflammatory state changes what the compound does.","description":"Stevioside at 1 mM suppressed LPS-induced TNF and IL-1beta release in human THP-1 cells, with suppression of IKKbeta and NF-kappaB activation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"06e03b41-c225-5fa3-8aa3-13a07a9826a9","slug":"stevioside","display_name":"Stevioside","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3d8d3010-7667-5664-b73d-e9f7199d6c10","slug":"human-thp1-lps-cytokine-release","display_name":"Human THP-1 LPS-induced cytokine release","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c78086c3-7657-589b-b353-1691df2d9f8f","slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"600d5b09-e092-5146-af1e-2510cf17bac6","slug":"tnf","display_name":"Tumor necrosis factor","entity_type_key":"cytokine"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e9fa1ab4-707e-5ff9-9d60-11f503b54c58","slug":"il1b","display_name":"Interleukin-1 beta / IL1B","entity_type_key":"cytokine"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"4d74dcb7-2df2-5a16-affe-bafd34db88dd","slug":"ikbkb","display_name":"Human I-kappa-B kinase beta / IKBKB","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human monocyte-derived cell line with LPS stimulation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"High direct exposure is not demonstrated after dietary sweetener use; pathway inhibition does not establish direct protein binding.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The inflammatory state changes what the compound does.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Anti-Inflammatory and Immunomodulatory Activities of Stevioside and Its Metabolite Steviol on THP-1 Cells. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16448183/ · DOI 10.1021/jf0523465","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"19e6f0fd-2f16-59c1-bf19-05e5db778148","evidence_kind":"source_excerpt","locator":"Lines 322-328","start_line":322,"end_line":328,"excerpt":"## stevia-immune-stimulated\nThe inflammatory state changes what the compound does.\nStevioside at 1 mM suppressed LPS-induced TNF and IL-1beta release in human THP-1 cells, with suppression of IKKbeta and NF-kappaB activation.\nModel: Human monocyte-derived cell line with LPS stimulation.\nLimitations: High direct exposure is not demonstrated after dietary sweetener use; pathway inhibition does not establish direct protein binding.\nEvidence access: Primary abstract\nAnti-Inflammatory and Immunomodulatory Activities of Stevioside and Its Metabolite Steviol on THP-1 Cells. · 2006 · https://pubmed.ncbi.nlm.nih.gov/16448183/ · DOI 10.1021/jf0523465","model_system":"Human monocyte-derived cell line with LPS stimulation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"295304c9-609c-5b05-a18f-a00ab7b965ed","stable_key":"import-6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7","title":"Stevia: glycoside metabolism, taste, ion channels and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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