{"id":"215281c7-50dd-5404-a858-cbc1ec0b31ab","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:gsh-gssa","predicate":"reported_relationship","statement":"Allicin reacts with glutathione to form GSSA, distinct from GSSG.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"51127d93-fefb-5369-b0c3-b551fb135fb8","mechanism_event_label":"Allicin reacts with glutathione to form GSSA, distinct from GSSG.","subject":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"object":{"id":"abc6eee3-1846-5180-9cc9-93379e6a02c9","slug":"s-allylmercaptoglutathione","display_name":"S-Allylmercaptoglutathione / GSSA","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"51127d93-fefb-5369-b0c3-b551fb135fb8","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:gsh-gssa-event","event_type":"biochemical_relationship","label":"Allicin reacts with glutathione to form GSSA, distinct from GSSG.","description":"**Thioallylation chemistry.** Allicin reacts with accessible cysteine thiols to form allyl-containing mixed disulfides. For a protein, the simplified first reaction is protein-SH + allicin → protein-S-S-allyl + allylsulfenic acid. Glutathione forms S-allylmercaptoglutathione (GSSA), which is chemically distinct from oxidized glutathione (GSSG). Accessibility, local environment, competing thiols, and exposure determine which targets are modified. An adduct alone does not demonstrate inhibition, phenotype mediation, or dietary target engagement. [Rabinkov 1998](https://pubmed.ncbi.nlm.nih.gov/9528659/) [Horn 2018](https://pmc.ncbi.nlm.nih.gov/articles/PMC6070820/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"as reported","sequence_order":0,"notes":""},{"entity":{"id":"abc6eee3-1846-5180-9cc9-93379e6a02c9","slug":"s-allylmercaptoglutathione","display_name":"S-Allylmercaptoglutathione / GSSA","entity_type_key":"small_molecule"},"role":"measured outcome","stoichiometry":null,"state_label":"not_reported","sequence_order":1,"notes":""},{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"reacting thiol substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cell-free product preparation and LC-MS characterization; not human oral metabolism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Allicin reacts with glutathione to form GSSA, distinct from GSSG.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"<i>S</i>-allylmercaptoglutathione Is a Substrate for Glutathione Reductase (E.C. 1.8.1.7) from Yeast (<i>Saccharomyces cerevisiae</i>). | 2018 | DOI 10.3390/antiox7070086 | PMID 29986384 | https://pubmed.ncbi.nlm.nih.gov/29986384/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC6070820/ | https://doi.org/10.3390/antiox7070086","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 39-39; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"680a6f8b-bdf2-5638-9fc7-cad6bf4bff2c","evidence_kind":"source_excerpt","locator":"Lines 39-39","start_line":39,"end_line":39,"excerpt":"**Thioallylation chemistry.** Allicin reacts with accessible cysteine thiols to form allyl-containing mixed disulfides. For a protein, the simplified first reaction is protein-SH + allicin → protein-S-S-allyl + allylsulfenic acid. Glutathione forms S-allylmercaptoglutathione (GSSA), which is chemically distinct from oxidized glutathione (GSSG). Accessibility, local environment, competing thiols, and exposure determine which targets are modified. An adduct alone does not demonstrate inhibition, phenotype mediation, or dietary target engagement. [Rabinkov 1998](https://pubmed.ncbi.nlm.nih.gov/9528659/) [Horn 2018](https://pmc.ncbi.nlm.nih.gov/articles/PMC6070820/)","model_system":"Cell-free product preparation and LC-MS characterization; not human oral metabolism.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"10820a6b-594a-547d-97f9-906ab4cc1d6e","stable_key":"import-c836a883-ac18-5eb2-9971-2f0b542feba8","title":"Allicin: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"2475d3eb681100a0a34577a47b7cba5b251df866fbd6ce795122ccabee360018","revision_id":"590df96d-2ed1-5763-b04c-bf0e096e603c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}