{"id":"20f18afd-85b2-51d1-9cf3-fd1acbbfb85b","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:pituitary-prl-down","predicate":"decreases_in_recorded_experiment","statement":"Prolactin decreased in the picomolar zinc-thymulin isolated rat anterior-pituitary experiment.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"807fcdd3-870b-56b5-bb09-ed78c406be92","mechanism_event_label":"Prolactin decreased in the picomolar zinc-thymulin isolated rat anterior-pituitary experiment.","subject":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"object":{"id":"7cf93432-2cca-543d-8c73-6d2229c9300f","slug":"rat-pituitary-prolactin-release","display_name":"Rat anterior-pituitary prolactin release","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"807fcdd3-870b-56b5-bb09-ed78c406be92","stable_key":"18100170-a014-51c3-adeb-9dab4162b8cc:pituitary-prl-down-event","event_type":"experimental_observation","label":"Prolactin decreased in the picomolar zinc-thymulin isolated rat anterior-pituitary experiment.","description":"In isolated rat anterior pituitary tissue, picomolar zinc–thymulin stimulated ACTH, accompanied by cAMP changes and cGMP changes at higher tested concentrations. A cAMP-directed phosphodiesterase inhibitor enhanced some responses, whereas the cGMP-directed inhibitor attenuated responses at higher concentrations. LH increased at selected concentrations, **GH did not**, and prolactin decreased. This does not establish human cortisol elevation or blood–brain barrier passage. [1997 study](https://pubmed.ncbi.nlm.nih.gov/9483196/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"23819462-9bdf-5856-beac-68b3dca9cf85","slug":"thymulin","display_name":"Thymulin","entity_type_key":"peptide"},"role":"tested factor","stoichiometry":null,"state_label":"Picomolar zinc-thymulin","sequence_order":0,"notes":""},{"entity":{"id":"7cf93432-2cca-543d-8c73-6d2229c9300f","slug":"rat-pituitary-prolactin-release","display_name":"Rat anterior-pituitary prolactin release","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"peptide-associated metal","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ad0ccd02-b864-5373-b21e-85aa6d912f73","slug":"intracellular-camp-content","display_name":"Intracellular cyclic AMP content","entity_type_key":"cellular_process"},"role":"associated messenger","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary bibliographic record and abstract checked; full methods/figures not comprehensively reviewed.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Picomolar zinc-thymulin\", \"comparator\": \"Matched tissue control\", \"endpoint\": \"Prolactin decreased in the picomolar zinc-thymulin isolated rat anterior-pituitary experiment.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Isolated rat anterior-pituitary tissue; picomolar zinc-thymulin; concentration-dependent cAMP/cGMP responses. Not human cortisol measurement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Interpret only within the recorded preparation, exposure and comparator. The complete source passage retains qualifications; unspecified doses/timing have not been extracted here. No clinical efficacy, nutrient deficiency or unique molecular mediation is inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Prolactin decreased in the picomolar zinc-thymulin isolated rat anterior-pituitary experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Thymulin stimulates corticotrophin release and cyclic nucleotide formation in the rat anterior pituitary gland. | 1997 | DOI 10.1159/000097322 | PMID 9483196 | https://pubmed.ncbi.nlm.nih.gov/9483196/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 104-104; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cb30bde9-07d7-5624-bb94-71e7c100921a","evidence_kind":"source_excerpt","locator":"Lines 104-104","start_line":104,"end_line":104,"excerpt":"In isolated rat anterior pituitary tissue, picomolar zinc–thymulin stimulated ACTH, accompanied by cAMP changes and cGMP changes at higher tested concentrations. A cAMP-directed phosphodiesterase inhibitor enhanced some responses, whereas the cGMP-directed inhibitor attenuated responses at higher concentrations. LH increased at selected concentrations, **GH did not**, and prolactin decreased. This does not establish human cortisol elevation or blood–brain barrier passage. [1997 study](https://pubmed.ncbi.nlm.nih.gov/9483196/)","model_system":"Isolated rat anterior-pituitary tissue; picomolar zinc-thymulin; concentration-dependent cAMP/cGMP responses. Not human cortisol measurement.","directness":"reported_statement","verification_status":"source_recorded","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e1871d49-9c6b-547b-a1bb-6e2b9270ca89","stable_key":"import-18100170-a014-51c3-adeb-9dab4162b8cc","title":"Thymulin: detailed mechanisms of action (reviewed 3 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"6e8ed0d7a5e23fc822f2ab418e0c4fb2411c01fcaa330039e8d7d93218cacc88","revision_id":"8c0e3449-9bbd-57c0-bb5e-de83f044f2ac","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[{"id":"bd0e7c90-69c4-5be4-9606-f18344c39b8b","title":"Rat pituitary prolactin: decreases versus increases after thymulin","kind":"contradiction","status":"open","why":"Two primary studies report opposite prolactin directions in different rat pituitary preparations and concentration regimes. This is a real cross-study comparison, not a correction of the earlier draft. Concentration and preparation are plausible explanations, not established resolution.","resolution":"Unresolved across studies. Preserve both measured directions and test matched concentration-response curves, preparation and age before proposing a universal feedback sign.","created_at":"2026-10-04 03:17:09","record_type":"conflict","display_label":"Recorded conflict","record_url":"/conflicts/bd0e7c90-69c4-5be4-9606-f18344c39b8b","sides":[{"conflict_id":"bd0e7c90-69c4-5be4-9606-f18344c39b8b","ordinal":0,"label":"1997 isolated pituitary tissue, picomolar exposure","revision_id":"8c0e3449-9bbd-57c0-bb5e-de83f044f2ac","start_line":104,"end_line":104,"quote":"In isolated rat anterior pituitary tissue, picomolar zinc–thymulin stimulated ACTH, accompanied by cAMP changes and cGMP changes at higher tested concentrations. A cAMP-directed phosphodiesterase inhibitor enhanced some responses, whereas the cGMP-directed inhibitor attenuated responses at higher concentrations. LH increased at selected concentrations, **GH did not**, and prolactin decreased. This does not establish human cortisol elevation or blood–brain barrier passage. [1997 study](https://pubmed.ncbi.nlm.nih.gov/9483196/)","source_key":"import-18100170-a014-51c3-adeb-9dab4162b8cc","source_title":"Thymulin: detailed mechanisms of action (reviewed 3 October 2026)","claim_ids":["20f18afd-85b2-51d1-9cf3-fd1acbbfb85b"]},{"conflict_id":"bd0e7c90-69c4-5be4-9606-f18344c39b8b","ordinal":1,"label":"1998 pituitary incubation experiment, concentration/age dependence","revision_id":"8c0e3449-9bbd-57c0-bb5e-de83f044f2ac","start_line":108,"end_line":108,"quote":"Likewise, a separate study found **prolactin and TSH increases**, with a bell-shaped concentration response in incubation experiments, potentiation by TRH and cAMP-associated interventions, and weaker responses in senescent preparations. EGTA blocked release. The opposite prolactin directions across studies are an unresolved comparison involving concentration and preparation, not grounds to discard either result. TRH-like behavior does not establish binding to the TRH receptor. [1998 prolactin/TSH study](https://pubmed.ncbi.nlm.nih.gov/9818729/)","source_key":"import-18100170-a014-51c3-adeb-9dab4162b8cc","source_title":"Thymulin: detailed mechanisms of action (reviewed 3 October 2026)","claim_ids":["15a6b592-94ca-5b7e-aec0-6c8d657bc378"]}]}],"corrections":[],"research":null}