{"id":"20e8306b-9e9e-55da-8dfd-e58fb753fefd","stable_key":"research:selenoh-associates-with-stress-dna","predicate":"binds","statement":"SELENOH showed redox-responsive association with stress-responsive DNA sequences.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"neutral","is_public":true,"mechanism_event_id":"ef6d778d-2a73-5a8a-b0d3-e1b3f32c7b5a","mechanism_event_label":"SELENOH can associate with DNA in a redox-sensitive manner.","subject":{"id":"207e9d1c-e66d-5349-ac6a-ef1ca1b31a32","slug":"selenoh","display_name":"SELENOH","entity_type_key":"protein"},"object":{"id":"5615b7b8-98c6-5ebe-95fe-6368bf0fe139","slug":"stress-responsive-dna","display_name":"Stress-responsive DNA elements","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"ef6d778d-2a73-5a8a-b0d3-e1b3f32c7b5a","stable_key":"research:selenoh-associates-with-stress-dna","event_type":"binding","label":"SELENOH can associate with DNA in a redox-sensitive manner.","description":"SELENOH showed redox-responsive association with stress-responsive DNA sequences.","status":"active","compartment":{"slug":"nucleus","display_name":"Nucleus"},"participants":[{"entity":{"id":"207e9d1c-e66d-5349-ac6a-ef1ca1b31a32","slug":"selenoh","display_name":"SELENOH","entity_type_key":"protein"},"role":"binding-protein","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5615b7b8-98c6-5ebe-95fe-6368bf0fe139","slug":"stress-responsive-dna","display_name":"Stress-responsive DNA elements","entity_type_key":"chemical_species"},"role":"binding-target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"HT22","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"DNA-binding and expression assays","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not a universal genomic target map.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"human protein in mouse cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"644b3242-c6f0-554e-b643-e05fab68bf85","evidence_kind":"curated_literature_summary","locator":"lines 858-868","start_line":858,"end_line":868,"excerpt":"## selenoh-associates-with-stress-dna\n\nSELENOH can associate with DNA in a redox-sensitive manner.\n\nSELENOH showed redox-responsive association with stress-responsive DNA sequences.\n\nOrganism: human protein in mouse cells\nCell type: HT22\nExperimental model: DNA-binding and expression assays\nLimitations: Not a universal genomic target map.\nPrimary reference: [Selenoprotein H is a redox-sensing high mobility group family DNA-binding protein that up-regulates genes involved in glutathione synthesis and phase II detoxification](https://pubmed.ncbi.nlm.nih.gov/17526492/)","model_system":"DNA-binding and expression assays","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"transcriptional-redox-regulation","plain_language":"SELENOH can associate with DNA in a redox-sensitive manner.","evidence_scope":"direct_experimental","papers":[{"key":"panee-2007-selenoh-dna","title":"Selenoprotein H is a redox-sensing high mobility group family DNA-binding protein that up-regulates genes involved in glutathione synthesis and phase II detoxification","url":"https://pubmed.ncbi.nlm.nih.gov/17526492/","doi":"10.1074/jbc.M702267200","year":2007,"model":"Human SELENOH expression in murine HT22 cells and DNA-binding assays","summary":"Tests redox-responsive DNA association and changes in glutathione-related gene expression."}]}}