{"id":"20ae22e9-79c2-59f4-95fd-b51bd4a8754b","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-gtpbp3-gtpase","predicate":"hydrolyzes","statement":"Purified mature human GTPBP3 exhibited GTPase activity, and functional experiments linked that activity to tRNA modification.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"357947f4-174d-54fd-927b-565b5f921c4a","mechanism_event_label":"The modifying complex includes a GTP-powered component.","subject":{"id":"cd2a6b5d-a6cf-5829-b0e4-b52a0f92d153","slug":"gtpbp3","display_name":"Human mitochondrial GTP-binding protein 3 / GTPBP3","entity_type_key":"protein"},"object":{"id":"c8ccc03a-29cc-5127-baf5-81eeb92ac780","slug":"gtp","display_name":"Guanosine triphosphate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"357947f4-174d-54fd-927b-565b5f921c4a","stable_key":"1acdb1e8-2159-5c7f-b39f-0153ff8024ae:taurine-gtpbp3-gtpase-event","event_type":"observed_relationship","label":"The modifying complex includes a GTP-powered component.","description":"Purified mature human GTPBP3 exhibited GTPase activity, and functional experiments linked that activity to tRNA modification.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"cd2a6b5d-a6cf-5829-b0e4-b52a0f92d153","slug":"gtpbp3","display_name":"Human mitochondrial GTP-binding protein 3 / GTPBP3","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"c8ccc03a-29cc-5127-baf5-81eeb92ac780","slug":"gtp","display_name":"Guanosine triphosphate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"e4ce9b58-a2a4-5ec2-a449-c529c360fb32","slug":"mto1","display_name":"Human mitochondrial tRNA modification protein / MTO1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e423cd36-f183-522b-a5e4-5f9ef50eee7e","slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human enzyme kinetics and cellular/yeast functional studies.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Results depend on the mature protein construct; do not infer benefit from dietary nucleotide supplements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Taurine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"taurine","display_name":"Taurine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The modifying complex includes a GTP-powered component.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The human tRNA taurine modification enzyme GTPBP3 is an active GTPase linked to mitochondrial diseases. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33619562/ · DOI 10.1093/nar/gkab104","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f4837f35-9678-561d-8ebb-77721613bdad","evidence_kind":"source_excerpt","locator":"Lines 265-271","start_line":265,"end_line":271,"excerpt":"## taurine-gtpbp3-gtpase\nThe modifying complex includes a GTP-powered component.\nPurified mature human GTPBP3 exhibited GTPase activity, and functional experiments linked that activity to tRNA modification.\nModel: Human enzyme kinetics and cellular/yeast functional studies.\nLimitations: Results depend on the mature protein construct; do not infer benefit from dietary nucleotide supplements.\nEvidence access: Primary abstract\nThe human tRNA taurine modification enzyme GTPBP3 is an active GTPase linked to mitochondrial diseases. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33619562/ · DOI 10.1093/nar/gkab104","model_system":"Human enzyme kinetics and cellular/yeast functional studies.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc8d9ab8-fdf9-5107-877c-336cf8005097","stable_key":"import-1acdb1e8-2159-5c7f-b39f-0153ff8024ae","title":"Taurine: synthesis, transport, mitochondrial decoding and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"c6cb77619a374d9b1b04830032fdd3b33d269d160df5baba3778fad5adf57db5","revision_id":"cc45c491-cc6a-5426-ab72-d7c6d26c3614","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}