{"id":"203a161a-ef79-52bc-9bb2-23253fc9932d","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-smct1-uptake","predicate":"imports","statement":"Expressing human intestinal SLC5A8 in Xenopus oocytes increased butyrate uptake and generated sodium-dependent inward currents.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"1a02dc7d-68f7-5a38-bf22-4fe0c356c0e8","mechanism_event_label":"A human transporter can concentrate butyrate using a sodium gradient.","subject":{"id":"4f815747-b853-574c-ae08-8400cb72fdc8","slug":"slc5a8","display_name":"Human sodium-coupled monocarboxylate transporter 1 / SLC5A8","entity_type_key":"protein"},"object":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"1a02dc7d-68f7-5a38-bf22-4fe0c356c0e8","stable_key":"104835a3-b891-5dc4-a6bb-42ab7c6f96c4:butyrate-smct1-uptake-event","event_type":"observed_relationship","label":"A human transporter can concentrate butyrate using a sodium gradient.","description":"Expressing human intestinal SLC5A8 in Xenopus oocytes increased butyrate uptake and generated sodium-dependent inward currents.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4f815747-b853-574c-ae08-8400cb72fdc8","slug":"slc5a8","display_name":"Human sodium-coupled monocarboxylate transporter 1 / SLC5A8","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e7428a7f-d35a-5406-9904-a88984814833","slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e565c195-2dc6-55b2-82df-0759aaaa11c6","slug":"human-slc5a8-butyrate-uptake","display_name":"Human SLC5A8-mediated butyrate uptake","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human SLC5A8 expressed in frog oocytes; radiotracer and voltage-clamp assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Expression host is not the protein species. This does not establish that extra dietary sodium improves uptake.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Butyrate collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"butyrate","display_name":"Butyrate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A human transporter can concentrate butyrate using a sodium gradient.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Functional identification of SLC5A8, a tumor suppressor down-regulated in colon cancer, as a Na(+)-coupled transporter for short-chain fatty acids. · 2004 · https://pubmed.ncbi.nlm.nih.gov/14966140/ · DOI 10.1074/jbc.C400059200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"raises","comparator":null,"unit":null,"notes":"Expression increased butyrate uptake and generated sodium-dependent inward currents.","entity":null},{"dimension":"transport_pool","value_text":"the expressing cell","comparator":null,"unit":null,"notes":"Expression increased butyrate uptake and generated sodium-dependent inward currents.","entity":null}],"evidence":[{"id":"0faf61e5-784c-515b-b1f8-41ba2c24698b","evidence_kind":"source_excerpt","locator":"Lines 102-108","start_line":102,"end_line":108,"excerpt":"## butyrate-smct1-uptake\nA human transporter can concentrate butyrate using a sodium gradient.\nExpressing human intestinal SLC5A8 in Xenopus oocytes increased butyrate uptake and generated sodium-dependent inward currents.\nModel: Human SLC5A8 expressed in frog oocytes; radiotracer and voltage-clamp assays.\nLimitations: Expression host is not the protein species. This does not establish that extra dietary sodium improves uptake.\nEvidence access: Primary abstract\nFunctional identification of SLC5A8, a tumor suppressor down-regulated in colon cancer, as a Na(+)-coupled transporter for short-chain fatty acids. · 2004 · https://pubmed.ncbi.nlm.nih.gov/14966140/ · DOI 10.1074/jbc.C400059200","model_system":"Human SLC5A8 expressed in frog oocytes; radiotracer and voltage-clamp assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"33205bb8-dcbe-5d2c-afca-86075d7748d1","stable_key":"import-104835a3-b891-5dc4-a6bb-42ab7c6f96c4","title":"Butyrate: microbial production, fuel use, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"a1253eb4426fc45d7b7c113028aa4ba2e9f89a9653272f3795437fc222bad558","revision_id":"c2320e65-a252-57a9-8838-7abf3d23400e","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}