{"id":"201095f3-4449-53ba-a823-cf197e56800d","stable_key":"195e8b84-ca5a-591a-a045-c55c53c8a01c:sodium-th17-p38","predicate":"required_in_test_for","statement":"Silencing or chemical inhibition of p38/MAPK blocked the high-salt enhancement of TH17 differentiation in the study.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fbbe0c89-229d-56e2-9351-0058a96e3094","mechanism_event_label":"Blocking p38/MAPK interrupted this particular salt-sensitive immune response.","subject":{"id":"8e62ed16-0010-5b56-acf2-f0684433098d","slug":"p38-mapk","display_name":"p38 mitogen-activated protein kinase family","entity_type_key":"protein_family"},"object":{"id":"0f810574-98a6-5395-a1c5-23e782d26603","slug":"th17-differentiation","display_name":"Th17 differentiation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"fbbe0c89-229d-56e2-9351-0058a96e3094","stable_key":"195e8b84-ca5a-591a-a045-c55c53c8a01c:sodium-th17-p38-event","event_type":"biochemical_relationship","label":"Blocking p38/MAPK interrupted this particular salt-sensitive immune response.","description":"Silencing or chemical inhibition of p38/MAPK blocked the high-salt enhancement of TH17 differentiation in the study.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f3fe99b8-4e4a-57cd-9a86-3651092c9f6c","slug":"sodium-chloride","display_name":"Sodium chloride","entity_type_key":"chemical_species"},"role":"experimental_exposure","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8e62ed16-0010-5b56-acf2-f0684433098d","slug":"p38-mapk","display_name":"p38 mitogen-activated protein kinase family","entity_type_key":"protein_family"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"0f810574-98a6-5395-a1c5-23e782d26603","slug":"th17-differentiation","display_name":"Th17 differentiation","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/sodium-research/23467095.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"d16bb4b4becde85238492e01054f360aeaf57ea90015cba99459cd155fa4251f\", \"start_char\": 0, \"end_char\": 1913, \"text_sha256\": \"d16bb4b4becde85238492e01054f360aeaf57ea90015cba99459cd155fa4251f\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cytokine-driven T-cell culture, gene silencing/inhibition and experimental autoimmune encephalomyelitis","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Increased NaCl in culture or high-salt mouse diet","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"NaCl exposure includes both ions and osmotic context. Mouse EAE and cultured T cells do not establish that salt causes human multiple sclerosis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Sodium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"sodium","display_name":"Sodium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human and mouse T cells; mouse disease model","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Blocking p38/MAPK interrupted this particular salt-sensitive immune response.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[sodium-p23467095] Sodium chloride drives autoimmune disease by the induction of pathogenic TH17 cells. (2013). https://pubmed.ncbi.nlm.nih.gov/23467095/ DOI: 10.1038/nature11868","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"T-helper cells and experimental CNS autoimmunity","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"df27cab3-2e77-5be0-bad5-2b68a5a05633","evidence_kind":"source_excerpt","locator":"Lines 499-510","start_line":499,"end_line":510,"excerpt":"### sodium-th17-p38\nSilencing or chemical inhibition of p38/MAPK blocked the high-salt enhancement of TH17 differentiation in the study.\nCondition category: normal\nnutrient_topic: Sodium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Blocking p38/MAPK interrupted this particular salt-sensitive immune response.\norganism: Human and mouse T cells; mouse disease model\ntissue_or_cell_type: T-helper cells and experimental CNS autoimmunity\nexperimental_model: Cytokine-driven T-cell culture, gene silencing/inhibition and experimental autoimmune encephalomyelitis\nlimitations: NaCl exposure includes both ions and osmotic context. Mouse EAE and cultured T cells do not establish that salt causes human multiple sclerosis.\nexposure: Increased NaCl in culture or high-salt mouse diet\nevidence_span: {\"source_cache\": \"artifacts/sodium-research/23467095.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"d16bb4b4becde85238492e01054f360aeaf57ea90015cba99459cd155fa4251f\", \"start_char\": 0, \"end_char\": 1913, \"text_sha256\": \"d16bb4b4becde85238492e01054f360aeaf57ea90015cba99459cd155fa4251f\"}\n[sodium-p23467095] Sodium chloride drives autoimmune disease by the induction of pathogenic TH17 cells. (2013). https://pubmed.ncbi.nlm.nih.gov/23467095/ DOI: 10.1038/nature11868","model_system":"Cytokine-driven T-cell culture, gene silencing/inhibition and experimental autoimmune encephalomyelitis","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [sodium-p23467095] Sodium chloride drives autoimmune disease by the induction of pathogenic TH17 cells. (2013). https://pubmed.ncbi.nlm.nih.gov/23467095/ DOI: 10.1038/nature11868","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"c260cd7b-beb0-5113-a198-69ec8fd4492c","stable_key":"import-195e8b84-ca5a-591a-a045-c55c53c8a01c","title":"Sodium: gradients, nutrient transport, fluid regulation and loss states (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"684f65cf0661c18395831ade4b02bb24d5e66c568f0d00a3471600eaf5d4d1c6","revision_id":"39866f04-3052-5f59-bd95-6806c5490875","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}