{"id":"1fe430a7-f099-5c16-a234-362413f1aad3","stable_key":"475ea55a-65e9-51c3-a539-738a6a8f683f:mangiferin-calcium-gsh","predicate":"increases","statement":"Calcium-challenged mitochondria from treated rats showed glutathione depletion.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"71055510-e213-52f5-970b-5d11f2c122b9","mechanism_event_label":"The calcium challenge changed the redox consequences.","subject":{"id":"2cfc0bd9-b5cc-58ab-852a-7705f3d8d47f","slug":"mangiferin","display_name":"Mangiferin","entity_type_key":"small_molecule"},"object":{"id":"1971ea50-6457-56d5-b817-c39a93fcdba6","slug":"rat-mitochondrial-gsh-oxidation","display_name":"Rat liver mitochondrial glutathione oxidation or depletion","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"71055510-e213-52f5-970b-5d11f2c122b9","stable_key":"475ea55a-65e9-51c3-a539-738a6a8f683f:mangiferin-calcium-gsh-event","event_type":"biochemical_relationship","label":"The calcium challenge changed the redox consequences.","description":"Calcium-challenged mitochondria from treated rats showed glutathione depletion.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b44c9e27-4bbb-52d3-a022-14cddded5073","slug":"glutathione","display_name":"GSH","entity_type_key":"small_molecule"},"role":"affected_buffer","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"trigger","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2cfc0bd9-b5cc-58ab-852a-7705f3d8d47f","slug":"mangiferin","display_name":"Mangiferin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"1971ea50-6457-56d5-b817-c39a93fcdba6","slug":"rat-mitochondrial-gsh-oxidation","display_name":"Rat liver mitochondrial glutathione oxidation or depletion","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/mangiferin-research/15979560.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"3de89f65f9561fd94c5f07121e8150f116c59277658d7e611a399dc2d963ec1f\", \"start_char\": 0, \"end_char\": 2234, \"text_sha256\": \"3de89f65f9561fd94c5f07121e8150f116c59277658d7e611a399dc2d963ec1f\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat pretreatment and isolated mitochondrial calcium challenge","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"40 mg/kg mangiferin pretreatment; isolated mitochondria exposed to calcium","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Mitochondrial challenge does not establish harm from usual oral intake; proposed quinone-thiol adduct mechanism was not fully proven.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Mangiferin research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"mangiferin","display_name":"Mangiferin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The calcium challenge changed the redox consequences.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mangiferin-p15979560] Mangiferin, a natural occurring glucosyl xanthone, increases susceptibility of rat liver mitochondria to calcium-induced permeability transition. 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