{"id":"1fc2a891-cff9-5a0e-bcf8-47ba276bdf3f","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-binding-residues","predicate":"has_mutation_sensitive_recognition_of","statement":"Mutagenesis identified eleven T1R2 residues important for aspartame responses: S40, Y103, D142, S144, S165, S168, Y215, D278, E302, D307 and R383.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"6ff8caec-eea7-5b61-a7e7-134f88fd4a77","mechanism_event_label":"Small changes in the sensor can alter recognition.","subject":{"id":"09713aab-d5e3-589c-83c2-95d3989efd62","slug":"tas1r2","display_name":"Human taste receptor TAS1R2","entity_type_key":"protein"},"object":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"6ff8caec-eea7-5b61-a7e7-134f88fd4a77","stable_key":"b428117d-3a8c-5e74-8711-168153e19545:aspartame-binding-residues-event","event_type":"observed_relationship","label":"Small changes in the sensor can alter recognition.","description":"Mutagenesis identified eleven T1R2 residues important for aspartame responses: S40, Y103, D142, S144, S165, S168, Y215, D278, E302, D307 and R383.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"09713aab-d5e3-589c-83c2-95d3989efd62","slug":"tas1r2","display_name":"Human taste receptor TAS1R2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"15bd1664-9d4b-514f-ab83-87274a67673f","slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7ab6f209-2089-5008-b64e-64f8db8c7771","slug":"tas1r3","display_name":"Human taste receptor TAS1R3","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"2eca6132-b441-5473-98bb-6759c0b2a5fc","slug":"human-tas1r2-tas1r3","display_name":"Human sweet taste receptor TAS1R2/TAS1R3","entity_type_key":"protein_complex"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human receptor mutants and homology models.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Loss of response can affect binding, folding or activation; not every residue is necessarily a direct contact.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Aspartame collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"aspartame","display_name":"Aspartame","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Small changes in the sensor can alter recognition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Characterization of the Binding Site of Aspartame in the Human Sweet Taste Receptor. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26377607/ · DOI 10.1093/chemse/bjv045","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6c9c4dfb-64ed-571c-b0fa-bb33968bda28","evidence_kind":"source_excerpt","locator":"Lines 170-176","start_line":170,"end_line":176,"excerpt":"## aspartame-binding-residues\nSmall changes in the sensor can alter recognition.\nMutagenesis identified eleven T1R2 residues important for aspartame responses: S40, Y103, D142, S144, S165, S168, Y215, D278, E302, D307 and R383.\nModel: Human receptor mutants and homology models.\nLimitations: Loss of response can affect binding, folding or activation; not every residue is necessarily a direct contact.\nEvidence access: Primary abstract\nCharacterization of the Binding Site of Aspartame in the Human Sweet Taste Receptor. · 2015 · https://pubmed.ncbi.nlm.nih.gov/26377607/ · DOI 10.1093/chemse/bjv045","model_system":"Human receptor mutants and homology models.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2bba7d36-993f-5ba7-acaf-2e4d573527ed","stable_key":"import-b428117d-3a8c-5e74-8711-168153e19545","title":"Aspartame: digestion, taste, metabolite dependencies and experimental signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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