{"id":"1fb0af02-80e5-5737-adaf-1b30e064e0b9","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:sensory-loss-mouse-trpa1","predicate":"decreases_in_recorded_experiment","statement":"Loss of mouse-trpa1 reduced but did not abolish allicin-evoked pain-related behavior.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"5c9680d2-5511-58a8-9af6-df2ee393dc20","mechanism_event_label":"Loss of mouse-trpa1 reduced but did not abolish allicin-evoked pain-related behavior.","subject":{"id":"05575212-6bc2-544a-8983-16b578525891","slug":"mouse-trpa1","display_name":"Mouse Trpa1 ion channel","entity_type_key":"protein"},"object":{"id":"9d05edbe-9a7d-529a-b9b6-d1ac2990e58a","slug":"mouse-allicin-pain-behavior","display_name":"Mouse pain-related behavior after allicin exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5c9680d2-5511-58a8-9af6-df2ee393dc20","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:sensory-loss-mouse-trpa1-event","event_type":"experimental_observation","label":"Loss of mouse-trpa1 reduced but did not abolish allicin-evoked pain-related behavior.","description":"**Sensory activation.** Experiments identified allicin-dependent activation of TRPA1 and TRPV1. In the later mouse study, allicin stimulated dorsal-root-ganglion neurons and pain-related behavior. Removing either Trpa1 or Trpv1 reduced, but did not abolish, the behavioral response. The channels provide overlapping sensory routes rather than one exclusive receptor. Irritation, chemical burns, and allergic dermatitis are different outcomes. [Macpherson 2005](https://pubmed.ncbi.nlm.nih.gov/15916949/) [Salazar 2008](https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"05575212-6bc2-544a-8983-16b578525891","slug":"mouse-trpa1","display_name":"Mouse Trpa1 ion channel","entity_type_key":"protein"},"role":"tested factor","stoichiometry":null,"state_label":"mouse-trpa1 deletion with allicin","sequence_order":0,"notes":""},{"entity":{"id":"9d05edbe-9a7d-529a-b9b6-d1ac2990e58a","slug":"mouse-allicin-pain-behavior","display_name":"Mouse pain-related behavior after allicin exposure","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""},{"entity":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"role":"sensory stimulus","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text available; selected claim-relevant methods, results, tables/figures and limitations reviewed. Supplemental proteome and all secondary findings are not exhaustively extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"present","comparator":"Wild-type with the same allicin exposure","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"deleted","comparator":"Wild-type with the same allicin exposure","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"mouse-trpa1","display_name":"Mouse Trpa1 ion channel","entity_type_key":"protein"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"mouse-trpa1 deletion with allicin\", \"comparator\": \"Wild-type with the same allicin exposure\", \"endpoint\": \"Loss of mouse-trpa1 reduced but did not abolish allicin-evoked pain-related behavior.\", \"effect_direction\": \"decrease\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"mouse-trpa1\", \"state\": \"deleted\"}, {\"entity_slug\": \"allicin\", \"state\": \"present\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Mouse single-gene knockout compared with its wild-type littermate background.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"No double-knockout inference; behavioral assay, not proof of human allergy or burns.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Loss of mouse-trpa1 reduced but did not abolish allicin-evoked pain-related behavior.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A single N-terminal cysteine in TRPV1 determines activation by pungent compounds from onion and garlic. | 2008 | DOI 10.1038/nn2056 | PMID 18297068 | https://pubmed.ncbi.nlm.nih.gov/18297068/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/ | https://doi.org/10.1038/nn2056","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 71-71; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"b0a598c8-c2da-55cc-aeab-003f4725363c","evidence_kind":"source_excerpt","locator":"Lines 71-71","start_line":71,"end_line":71,"excerpt":"**Sensory activation.** Experiments identified allicin-dependent activation of TRPA1 and TRPV1. In the later mouse study, allicin stimulated dorsal-root-ganglion neurons and pain-related behavior. Removing either Trpa1 or Trpv1 reduced, but did not abolish, the behavioral response. The channels provide overlapping sensory routes rather than one exclusive receptor. Irritation, chemical burns, and allergic dermatitis are different outcomes. [Macpherson 2005](https://pubmed.ncbi.nlm.nih.gov/15916949/) [Salazar 2008](https://pmc.ncbi.nlm.nih.gov/articles/PMC4370189/)","model_system":"Mouse single-gene knockout compared with its wild-type littermate background.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"10820a6b-594a-547d-97f9-906ab4cc1d6e","stable_key":"import-c836a883-ac18-5eb2-9971-2f0b542feba8","title":"Allicin: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"2475d3eb681100a0a34577a47b7cba5b251df866fbd6ce795122ccabee360018","revision_id":"590df96d-2ed1-5763-b04c-bf0e096e603c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}