{"id":"1f9d508e-f5e8-5da2-8838-df257f481a36","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-pump-two-occluded-sites","predicate":"occludes-potassium-congener-at-two-sites","statement":"The pig pump structure resolved two occluded rubidium ions at potassium transport sites in its alpha subunit.","claim_class":"identity","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"f3544846-3634-5aec-91cb-8d9eaa7e562a","mechanism_event_label":"The pump has two sites that can trap a potassium-like ion during transport.","subject":{"id":"27c3e7bd-c6e1-5344-8130-b231a9da2cfb","slug":"sodium-potassium-atpase","display_name":"Sodium-potassium ATPase complexes","entity_type_key":"protein_family"},"object":{"id":"8155f62e-a68f-5256-851d-30c33b497aaf","slug":"rubidium-ion","display_name":"Rubidium ion","entity_type_key":"ion"},"evidence_count":1,"mechanism_event":{"id":"f3544846-3634-5aec-91cb-8d9eaa7e562a","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:k-pump-two-occluded-sites-event","event_type":"biochemical_relationship","label":"The pump has two sites that can trap a potassium-like ion during transport.","description":"The pig pump structure resolved two occluded rubidium ions at potassium transport sites in its alpha subunit.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5dd31e52-f51e-51f3-880e-240abcc0ab1d","slug":"potassium-ion","display_name":"Potassium ion","entity_type_key":"ion"},"role":"physiological counterpart of crystallographic congener","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"27c3e7bd-c6e1-5344-8130-b231a9da2cfb","slug":"sodium-potassium-atpase","display_name":"Sodium-potassium ATPase complexes","entity_type_key":"protein_family"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"8155f62e-a68f-5256-851d-30c33b497aaf","slug":"rubidium-ion","display_name":"Rubidium ion","entity_type_key":"ion"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Purified pig renal Na,K-ATPase; 3.5 angstrom crystallography with rubidium.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Rubidium is a congener; a static purified structure is not a dietary depletion test.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Pig","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The pump has two sites that can trap a potassium-like ion during transport.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[morth-2007-pump] Crystal structure of the sodium-potassium pump (2007). https://pubmed.ncbi.nlm.nih.gov/18075585/ DOI: 10.1038/nature06419","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Renal membrane enzyme","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f1c6d9a9-8468-5768-bb9e-6df8628acc8e","evidence_kind":"source_excerpt","locator":"Lines 599-608","start_line":599,"end_line":608,"excerpt":"### k-pump-two-occluded-sites\nThe pig pump structure resolved two occluded rubidium ions at potassium transport sites in its alpha subunit.\nCondition category: normal\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The pump has two sites that can trap a potassium-like ion during transport.\norganism: Pig\ntissue_or_cell_type: Renal membrane enzyme\nexperimental_model: Purified pig renal Na,K-ATPase; 3.5 angstrom crystallography with rubidium.\nlimitations: Rubidium is a congener; a static purified structure is not a dietary depletion test.\n[morth-2007-pump] Crystal structure of the sodium-potassium pump (2007). https://pubmed.ncbi.nlm.nih.gov/18075585/ DOI: 10.1038/nature06419","model_system":"Purified pig renal Na,K-ATPase; 3.5 angstrom crystallography with rubidium.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [morth-2007-pump] Crystal structure of the sodium-potassium pump (2007). https://pubmed.ncbi.nlm.nih.gov/18075585/ DOI: 10.1038/nature06419","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"241226ee-6b7d-5cc7-9781-e6beb8ee1982","stable_key":"import-5102beb6-9f61-500e-af10-9ac63649e0b7","title":"Potassium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a8f89f8d84b48c0f93048dfc45f7f57a67dd9a7249968a096a14aeb03d8b7a52","revision_id":"de4ec6f4-58a1-537b-8169-97b783898df1","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}